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Home › Dataset Library › Establishment of objective criteria for selecting relevant intestinal cell-based models

Dataset: Establishment of objective criteria for selecting relevant intestinal cell-based models

The objective of this study was to make use of gene expression signatures and functional assays to delineate differences between various...

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The objective of this study was to make use of gene expression signatures and functional assays to delineate differences between various intestinal colon carcinoma cell lines and normal intestinal epithelium to assess their appropriateness as a tumor model or for drug absorption studies. [Cell lines] Total RNA of biological replicate samples (i.e.different passages) from a panel of cell lines was collected and resulting amplified cRNA hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays. Samples are labeled as follows: SampleNumber_CellLine. [Laser dissected tumor cells (LDM)] Total RNA of three different samples laser dissected tumor cells, normal colonocytes, and enterocytes of ileum and two samples of jejunum was collected and resulting amplified cRNA hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays. Samples are labeled as follows: SampleNumber_TissueType_Origin.

Species:
human

Samples:
43

Source:
E-GEOD-30292

Updated:
Dec.12, 2014

Registered:
Sep.16, 2014


Factors: (via ArrayExpress)
Sample GROWTH PROTOCOL CELL LINE CELL TYPE ORGANISM PART LASER-DISSECTED
GSM750842 3-week Caco-2 cells not specified colon not specified
GSM750842 3-week Caco-2 cells not specified colon not specified
GSM750842 3-week Caco-2 cells not specified colon not specified
GSM750845 3-week HT29 cells not specified colon not specified
GSM750845 3-week HT29 cells not specified colon not specified
GSM750845 3-week HT29 cells not specified colon not specified
GSM750848 3-week T84 cells not specified colon, metastatic site lung not specified
GSM750848 3-week T84 cells not specified colon, metastatic site lung not specified
GSM750848 3-week T84 cells not specified colon, metastatic site lung not specified
GSM75085 not specified SW480 cells not specified colon not specified
GSM75085 not specified SW480 cells not specified colon not specified
GSM75085 not specified SW480 cells not specified colon not specified
GSM750854 not specified LS174T cells not specified colon not specified
GSM750854 not specified LS174T cells not specified colon not specified
GSM750854 not specified LS174T cells not specified colon not specified
GSM750857 grown on filters Caco-2LD cells not specified colon not specified
GSM750857 grown on filters Caco-2LD cells not specified colon not specified
GSM750857 grown on filters Caco-2LD cells not specified colon not specified
GSM750860 not specified Caco-2 cells not specified colon not specified
GSM750842 3-week Caco-2 cells not specified colon not specified
GSM750862 grown on filters CacoReady cells not specified colon not specified
GSM750862 grown on filters CacoReady cells not specified colon not specified
GSM750862 grown on filters CacoReady cells not specified colon not specified
GSM750865 exposed to shipping media, grown on filters CacoReady cells not specified colon not specified
GSM750865 exposed to shipping media, grown on filters CacoReady cells not specified colon not specified
GSM750865 exposed to shipping media, grown on filters CacoReady cells not specified colon not specified
GSM750868 3-week , grown on filters CDX2-knockdown Caco-2 cells not specified colon not specified
GSM750868 3-week , grown on filters CDX2-knockdown Caco-2 cells not specified colon not specified
GSM750870 3-week , grown on filters negative control Caco-2 cells not specified colon not specified
GSM750879 not specified not specified tumor cells colon yes
GSM750879 not specified not specified tumor cells colon yes
GSM750879 not specified not specified tumor cells colon yes
GSM750882 not specified not specified normal colonocytes colon yes
GSM750882 not specified not specified normal colonocytes colon yes
GSM750882 not specified not specified normal colonocytes colon yes
GSM750885 not specified not specified primary cell cultures of cancer-associated fibroblasts colon no
GSM750885 not specified not specified primary cell cultures of cancer-associated fibroblasts colon no
GSM750885 not specified not specified primary cell cultures of cancer-associated fibroblasts colon no
GSM750888 not specified not specified normal enterocytes ileum yes
GSM750888 not specified not specified normal enterocytes ileum yes
GSM750888 not specified not specified normal enterocytes ileum yes
GSM75089 not specified not specified normal enterocytes jejunum yes
GSM75089 not specified not specified normal enterocytes jejunum yes

Tags

  • carcinoma
  • cell
  • colon
  • colon carcinoma
  • genome
  • ileum
  • intestinal epithelium
  • jejunum

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