<?xml version="1.0" encoding="ASCII"?>
<biogps><data><item key="owner">ArrayExpress Uploader</item><item key="pop_total">0</item><item key="species">human</item><item key="factors"><item><item key="GSM750842"><item key="GROWTH PROTOCOL">3-week</item><item key="CELL LINE">Caco-2 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750842"><item key="GROWTH PROTOCOL">3-week</item><item key="CELL LINE">Caco-2 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750842"><item key="GROWTH PROTOCOL">3-week</item><item key="CELL LINE">Caco-2 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750845"><item key="GROWTH PROTOCOL">3-week</item><item key="CELL LINE">HT29 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750845"><item key="GROWTH PROTOCOL">3-week</item><item key="CELL LINE">HT29 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750845"><item key="GROWTH PROTOCOL">3-week</item><item key="CELL LINE">HT29 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750848"><item key="GROWTH PROTOCOL">3-week</item><item key="CELL LINE">T84 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon, metastatic site lung</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750848"><item key="GROWTH PROTOCOL">3-week</item><item key="CELL LINE">T84 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon, metastatic site lung</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750848"><item key="GROWTH PROTOCOL">3-week</item><item key="CELL LINE">T84 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon, metastatic site lung</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM75085"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">SW480 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM75085"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">SW480 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM75085"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">SW480 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750854"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">LS174T cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750854"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">LS174T cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750854"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">LS174T cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750857"><item key="GROWTH PROTOCOL">grown on filters</item><item key="CELL LINE">Caco-2LD cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750857"><item key="GROWTH PROTOCOL">grown on filters</item><item key="CELL LINE">Caco-2LD cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750857"><item key="GROWTH PROTOCOL">grown on filters</item><item key="CELL LINE">Caco-2LD cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750860"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">Caco-2 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750842"><item key="GROWTH PROTOCOL">3-week</item><item key="CELL LINE">Caco-2 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750862"><item key="GROWTH PROTOCOL">grown on filters</item><item key="CELL LINE">CacoReady cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750862"><item key="GROWTH PROTOCOL">grown on filters</item><item key="CELL LINE">CacoReady cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750862"><item key="GROWTH PROTOCOL">grown on filters</item><item key="CELL LINE">CacoReady cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750865"><item key="GROWTH PROTOCOL">exposed to shipping media, grown on filters</item><item key="CELL LINE">CacoReady cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750865"><item key="GROWTH PROTOCOL">exposed to shipping media, grown on filters</item><item key="CELL LINE">CacoReady cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750865"><item key="GROWTH PROTOCOL">exposed to shipping media, grown on filters</item><item key="CELL LINE">CacoReady cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750868"><item key="GROWTH PROTOCOL">3-week , grown on filters</item><item key="CELL LINE">CDX2-knockdown Caco-2 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750868"><item key="GROWTH PROTOCOL">3-week , grown on filters</item><item key="CELL LINE">CDX2-knockdown Caco-2 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750870"><item key="GROWTH PROTOCOL">3-week , grown on filters</item><item key="CELL LINE">negative control Caco-2 cells</item><item key="CELL TYPE">not specified</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">not specified</item></item></item><item><item key="GSM750879"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">tumor cells</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">yes</item></item></item><item><item key="GSM750879"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">tumor cells</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">yes</item></item></item><item><item key="GSM750879"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">tumor cells</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">yes</item></item></item><item><item key="GSM750882"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">normal colonocytes</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">yes</item></item></item><item><item key="GSM750882"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">normal colonocytes</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">yes</item></item></item><item><item key="GSM750882"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">normal colonocytes</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">yes</item></item></item><item><item key="GSM750885"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">primary cell cultures of cancer-associated fibroblasts</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">no</item></item></item><item><item key="GSM750885"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">primary cell cultures of cancer-associated fibroblasts</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">no</item></item></item><item><item key="GSM750885"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">primary cell cultures of cancer-associated fibroblasts</item><item key="ORGANISM PART">colon</item><item key="LASER-DISSECTED">no</item></item></item><item><item key="GSM750888"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">normal enterocytes</item><item key="ORGANISM PART">ileum</item><item key="LASER-DISSECTED">yes</item></item></item><item><item key="GSM750888"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">normal enterocytes</item><item key="ORGANISM PART">ileum</item><item key="LASER-DISSECTED">yes</item></item></item><item><item key="GSM750888"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">normal enterocytes</item><item key="ORGANISM PART">ileum</item><item key="LASER-DISSECTED">yes</item></item></item><item><item key="GSM75089"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">normal enterocytes</item><item key="ORGANISM PART">jejunum</item><item key="LASER-DISSECTED">yes</item></item></item><item><item key="GSM75089"><item key="GROWTH PROTOCOL">not specified</item><item key="CELL LINE">not specified</item><item key="CELL TYPE">normal enterocytes</item><item key="ORGANISM PART">jejunum</item><item key="LASER-DISSECTED">yes</item></item></item></item><item key="id">4147</item><item key="ownerprofile_id">arrayexpress_sid</item><item key="platform">4</item><item key="summary_wrapped">The objective of this study was to make use of gene expression signatures and functional assays to delineate differences between various...</item><item key="geo_gse_id">E-GEOD-30292</item><item key="owner_profile">/profile/8773/arrayexpressuploader</item><item key="factor_count">5</item><item key="sample_count">43</item><item key="tags"><item>carcinoma</item><item>cell</item><item>colon</item><item>colon carcinoma</item><item>genome</item><item>ileum</item><item>intestinal epithelium</item><item>jejunum</item></item><item key="lastmodified">Dec.12, 2014</item><item key="is_default">False</item><item key="geo_gds_id"/><item key="slug">establishment-of-objective-criteria-for-selecting</item><item key="geo_id_plat">E-GEOD-30292_A-AFFY-44</item><item key="name">Establishment of objective criteria for selecting relevant intestinal cell-based models</item><item key="created">Sep.16, 2014</item><item key="summary">The objective of this study was to make use of gene expression signatures and functional assays to delineate differences between various intestinal colon carcinoma cell lines and normal intestinal epithelium to assess their appropriateness as a tumor model or for drug absorption studies. [Cell lines] Total RNA of biological replicate samples (i.e.different passages) from a  panel of cell lines was collected and resulting amplified cRNA hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays. Samples are labeled as follows: SampleNumber_CellLine.  [Laser dissected tumor cells (LDM)] Total RNA of three different samples laser dissected tumor cells, normal colonocytes, and enterocytes of ileum and two samples of jejunum was collected and resulting amplified cRNA hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays. Samples are labeled as follows: SampleNumber_TissueType_Origin.</item><item key="source">http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30292</item><item key="sample_source">http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30292/samples/</item></data></biogps>
