ArrayExpress Uploaderarrayexpress_sid6510NoneLow FatNoneLow FatNoneLow FatNoneLow FatFatty acid binding protein 4 promoter-Cre (FABP4-Cre)Low FatFatty acid binding protein 4 promoter-Cre (FABP4-Cre)Low FatFatty acid binding protein 4 promoter-Cre (FABP4-Cre)Low FatFatty acid binding protein 4 promoter-Cre (FABP4-Cre)Low FatNoneHigh FatNoneHigh FatNoneHigh FatNoneHigh FatFatty acid binding protein 4 promoter-Cre (FABP4-Cre)High FatFatty acid binding protein 4 promoter-Cre (FABP4-Cre)High FatFatty acid binding protein 4 promoter-Cre (FABP4-Cre)High FatFatty acid binding protein 4 promoter-Cre (FABP4-Cre)High Fat06Adipose tissue plays an important role in storing excess nutrients and preventing ectopic lipid accumulation in other organs. Obesity...22883230E-GEOD-30247/profile/8773/arrayexpressuploader216adipose tissuelipidobesityDec.12, 2014Falsehigh-fat-diet-triggers-sirt1-cleavage-in-adipose-tE-GEOD-30247_A-AFFY-45High Fat Diet Triggers SIRT1 Cleavage in Adipose Tissue Providing a Link between Dietary Stress and Metabolic Dysfunction.Nov.11, 2014Adipose tissue plays an important role in storing excess nutrients and preventing ectopic lipid accumulation in other organs. Obesity leads to excess lipid storage in adipocytes, resulting in the generation of stress signals and the derangement of metabolic functions. SIRT1 is an important regulatory sensor of nutrient availability in many metabolic tissues. Here we report that SIRT1 functions in adipose tissue to protect from the development of inflammation and obesity under normal feeding conditions, and the progression to metabolic dysfunction under dietary stress. Genetic ablation of SIRT1 from adipose tissue leads to gene expression changes that highly overlap with changes induced by high fat diet in wild type mice, suggesting that dietary stress signals inhibit the activity of SIRT1. Indeed, we show that high fat diet induces the cleavage of SIRT1 in adipose tissue by the inflammation-activated caspase-1, providing a link between dietary stress and predisposition to metabolic dysfunction. Four replicates from four different biological conditions: 1) SIRT1 wild-type fed low fat diet, 2) SIRT1 wild-type fed high fat diet, 3) SIRT1 knock-out fed low fat diet, 4) SIRT1 knock-out fed high fat diethttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30247mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30247/samples/