{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 4116, "factors": [{"GSM735094": {"APOE STATUS": "APOE e4+", "BRAAK STAGE": "Braak I-II"}}, {"GSM735094": {"APOE STATUS": "APOE e4+", "BRAAK STAGE": "Braak I-II"}}, {"GSM735094": {"APOE STATUS": "APOE e4+", "BRAAK STAGE": "Braak I-II"}}, {"GSM735097": {"APOE STATUS": "APOE e4-", "BRAAK STAGE": "Braak I-II"}}, {"GSM735097": {"APOE STATUS": "APOE e4-", "BRAAK STAGE": "Braak I-II"}}, {"GSM735097": {"APOE STATUS": "APOE e4-", "BRAAK STAGE": "Braak I-II"}}, {"GSM735100": {"APOE STATUS": "APOE e4+", "BRAAK STAGE": "Braak III-IV"}}, {"GSM735100": {"APOE STATUS": "APOE e4+", "BRAAK STAGE": "Braak III-IV"}}, {"GSM735100": {"APOE STATUS": "APOE e4+", "BRAAK STAGE": "Braak III-IV"}}, {"GSM735103": {"APOE STATUS": "APOE e4-", "BRAAK STAGE": "Braak III-IV"}}, {"GSM735103": {"APOE STATUS": "APOE e4-", "BRAAK STAGE": "Braak III-IV"}}, {"GSM735103": {"APOE STATUS": "APOE e4-", "BRAAK STAGE": "Braak III-IV"}}, {"GSM735106": {"APOE STATUS": "APOE e4+", "BRAAK STAGE": "Braak V-VI"}}, {"GSM735106": {"APOE STATUS": "APOE e4+", "BRAAK STAGE": "Braak V-VI"}}, {"GSM735106": {"APOE STATUS": "APOE e4+", "BRAAK STAGE": "Braak V-VI"}}, {"GSM735109": {"APOE STATUS": "APOE e4-", "BRAAK STAGE": "Braak V-VI"}}, {"GSM735109": {"APOE STATUS": "APOE e4-", "BRAAK STAGE": "Braak V-VI"}}, {"GSM735109": {"APOE STATUS": "APOE e4-", "BRAAK STAGE": "Braak V-VI"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Astrocyte dysfunction impacts their normal function, including neuronal support, thereby contributing to neurodegenerative pathologies...", "pubmed_id": 21705112, "geo_gse_id": "E-GEOD-29652", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 18, "tags": ["alzheimer's disease", "astrocyte", "brain", "cortex", "disease", "lcm"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "microarray-analysis-of-the-astrocyte-transcriptome", "geo_id_plat": "E-GEOD-29652_A-AFFY-44", "name": "Microarray analysis of the astrocyte transcriptome in the ageing brain: relationship to Alzheimer's pathology and ApoE genotype", "created": "Sep.16, 2014", "summary": "Astrocyte dysfunction impacts their normal function, including neuronal support, thereby contributing to neurodegenerative pathologies including Alzheimer's disease (AD). Therefore to understand the role of astrocytes in the pathogenesis of age-related disorders we analysed the gene expression profile of astrocytes with respect to Alzheimer-type pathology. The aim of the present study was to combine immuno-LCM and microarray analysis to characterise the astrocyte transcriptome at different Braak stages, and with respect to ApoE genotype, in post-mortem human temporal cortex sampled dervied from the Medical Research Council Cognitive Function and Ageing Study (MRC-CFAS). GFAP-positive astrocytes were isolated from age, sex and brain pH-matched cases derived from the MRC-CFAS cohort, using immuno laser capture microdissection. The extracted RNA was amplified and applied to HGU133 Plus 2.0 Affymetrix gene arrays. Genes were considered differentially expressed if they showed a minimum 1.5 fold change at p<0.02 in all 6 individual cases in each stage of pathology [18 cases in total: 6 cases Braak stages I-II (3 cases carried at least one ApoE epsilon4 allele and 3 were ApoEe4 negative); 6 cases Braak stages III-IV (3 ApoEe4 positive and 3 ApoEe4 negative; 6 cases Braak stages V-VI (3 ApoEe4 positive and 3 ApoEe4 negative)]. keywords: human GFAP-positive astrocytes", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29652", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29652/samples/"}