ArrayExpress Uploader0mousewild-typemalewild-typemalewild-typemaleHCMmaleHCMmaleHCMmalewild-typefemalewild-typefemaleHCMfemaleHCMfemale6487arrayexpress_sid6A soy diet worsens the progression of an inherited form of hypertrophic cardiomyopathy (HCM) in male mice when compared to casein-fed...E-GEOD-29648/profile/8773/arrayexpressuploader210cardiomyopathydiseasegenomehypertrophic cardiomyopathyleftDec.12, 2014Falsethe-impact-of-a-phytoestrogen-rich-diet-on-cardiacE-GEOD-29648_A-AFFY-45The impact of a phytoestrogen-rich diet on cardiac gene expression in the context of HCMNov.11, 2014A soy diet worsens the progression of an inherited form of hypertrophic cardiomyopathy (HCM) in male mice when compared to casein-fed mice. Females are largely resistant to this diet effect and better preserve cardiac function. We hypothesized that the abundant phytoestrogens found in soy are mainly responsible for this diet-dependent phenotype. Indeed, feeding male mice a phytoestrogen-supplemented casein-based diet can recapitulate the negative outcome seen when male mice are fed a standard soy-based diet. To gain mechanistic insights into disease pathogenesis, we used Affymetrix microarrays to profile gene expression in left ventricular tissue from 2 month old HCM male and female mice as well as wild-type littermate controls. These mice were fed a phytoestrogen (genistein + daidzein)-supplemented casein-based diet. We identified a number of molecular pathways that could explain both the male and female phenotypes. Hearts from male and female wild-type or HCM C57BL/6 mice fed a phytoestrogen-supplemented (genistein and daidzein mix) casein-based diet were excised at 2 months of age. Total RNA was extracted from left ventricles. Biotin-labeled amplified RNA was hybridized to Affymetrix Mouse Genome 430 2.0 microarrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29648http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29648/samples/