{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM735040": {"GENOTYPE": "wild-type", "SEX": "male"}}, {"GSM735040": {"GENOTYPE": "wild-type", "SEX": "male"}}, {"GSM735040": {"GENOTYPE": "wild-type", "SEX": "male"}}, {"GSM735043": {"GENOTYPE": "HCM", "SEX": "male"}}, {"GSM735043": {"GENOTYPE": "HCM", "SEX": "male"}}, {"GSM735043": {"GENOTYPE": "HCM", "SEX": "male"}}, {"GSM735046": {"GENOTYPE": "wild-type", "SEX": "female"}}, {"GSM735046": {"GENOTYPE": "wild-type", "SEX": "female"}}, {"GSM735048": {"GENOTYPE": "HCM", "SEX": "female"}}, {"GSM735048": {"GENOTYPE": "HCM", "SEX": "female"}}], "id": 6487, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "A soy diet worsens the progression of an inherited form of hypertrophic cardiomyopathy (HCM) in male mice when compared to casein-fed...", "geo_gse_id": "E-GEOD-29648", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 10, "tags": ["cardiomyopathy", "disease", "genome", "hypertrophic cardiomyopathy", "left"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "the-impact-of-a-phytoestrogen-rich-diet-on-cardiac", "geo_id_plat": "E-GEOD-29648_A-AFFY-45", "name": "The impact of a phytoestrogen-rich diet on cardiac gene expression in the context of HCM", "created": "Nov.11, 2014", "summary": "A soy diet worsens the progression of an inherited form of hypertrophic cardiomyopathy (HCM) in male mice when compared to casein-fed mice. Females are largely resistant to this diet effect and better preserve cardiac function. We hypothesized that the abundant phytoestrogens found in soy are mainly responsible for this diet-dependent phenotype. Indeed, feeding male mice a phytoestrogen-supplemented casein-based diet can recapitulate the negative outcome seen when male mice are fed a standard soy-based diet. To gain mechanistic insights into disease pathogenesis, we used Affymetrix microarrays to profile gene expression in left ventricular tissue from 2 month old HCM male and female mice as well as wild-type littermate controls. These mice were fed a phytoestrogen (genistein + daidzein)-supplemented casein-based diet. We identified a number of molecular pathways that could explain both the male and female phenotypes. Hearts from male and female wild-type or HCM C57BL/6 mice fed a phytoestrogen-supplemented (genistein and daidzein mix) casein-based diet were excised at 2 months of age. Total RNA was extracted from left ventricles. Biotin-labeled amplified RNA was hybridized to Affymetrix Mouse Genome 430 2.0 microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29648", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29648/samples/"}