{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM725964": {"DEVELOPMENTAL STAGE": "20.3wk fetus"}}, {"GSM725964": {"DEVELOPMENTAL STAGE": "20.3wk fetus"}}, {"GSM725966": {"DEVELOPMENTAL STAGE": "21.1wk fetus"}}, {"GSM725966": {"DEVELOPMENTAL STAGE": "21.1wk fetus"}}, {"GSM725968": {"DEVELOPMENTAL STAGE": "21wk fetus"}}, {"GSM725968": {"DEVELOPMENTAL STAGE": "21wk fetus"}}, {"GSM725970": {"DEVELOPMENTAL STAGE": "20wk fetus"}}, {"GSM725970": {"DEVELOPMENTAL STAGE": "20wk fetus"}}, {"GSM725972": {"DEVELOPMENTAL STAGE": "22.5wk fetus"}}, {"GSM725972": {"DEVELOPMENTAL STAGE": "22.5wk fetus"}}], "id": 4092, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Glial progenitor cells (GPCs) pervade the human brain. These cells express gangliosides recognized by MAb A2B5, and some but not all can...", "geo_gse_id": "E-GEOD-29368", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 10, "tags": ["astrocyte", "brain", "forebrain", "notch", "oligodendrocyte"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-29368_A-AFFY-44", "slug": "cd140a-human-glial-progenitor-cells", "geo_gds_id": "", "name": "CD140a+ human glial progenitor cells", "created": "Sep.16, 2014", "summary": "Glial progenitor cells (GPCs) pervade the human brain. These cells express gangliosides recognized by MAb A2B5, and some but not all can generate oligodendrocytes. Since some A2B5+ GPCs express PDGFa receptor (PDGFRa), which is critical to oligodendrocyte development, we asked if PDGFRa-directed sorting might isolate oligodendrocyte-competent progenitors. We used FACS to sort PDGFRa+ cells from the second trimester fetal human forebrain, based on expression of the PDGFRa epitope CD140a. CD140a+ cells could be maintained as mitotic progenitors that could be instructed to either oligodendrocyte or astrocyte phenotype. Transplanted CD140a+ cells robustly myelinated the hypomyelinated shiverer mouse brain. Microarray confirmed that CD140a+ cells differentially expressed PDGFRA, NG2, OLIG1/2, NKX2.2 and SOX2. Some expressed CD9, thereby defining a CD140a+/CD9+ fraction of oligodendrocyte-biased progenitors. CD140a+ cells differentially expressed genes of the PTN-PTPRZ1, wnt, notch and BMP pathways, suggesting the interaction of self-renewal and fate-restricting pathways in these cells, while identifying targets for their mobilization and instruction. 10 samples, 5 CD140a+, and 5 CD140a- sorted samples for individual fetal human brain", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29368", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29368/samples/"}