{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM723804": {"AGE": "4 weeks after birth", "CELL TYPE": "VSEL", "STRAIN OR LINE": "C57BL/6"}}, {"GSM723804": {"AGE": "4 weeks after birth", "CELL TYPE": "VSEL", "STRAIN OR LINE": "C57BL/6"}}, {"GSM723804": {"AGE": "4 weeks after birth", "CELL TYPE": "VSEL", "STRAIN OR LINE": "C57BL/6"}}, {"GSM723807": {"AGE": "4 weeks after birth", "CELL TYPE": "HSC", "STRAIN OR LINE": "C57BL/6"}}, {"GSM723807": {"AGE": "4 weeks after birth", "CELL TYPE": "HSC", "STRAIN OR LINE": "C57BL/6"}}, {"GSM723807": {"AGE": "4 weeks after birth", "CELL TYPE": "HSC", "STRAIN OR LINE": "C57BL/6"}}, {"GSM723810": {"AGE": "embryo before implantation", "CELL TYPE": "not specified", "STRAIN OR LINE": "129S2/SvPas"}}, {"GSM723810": {"AGE": "embryo before implantation", "CELL TYPE": "not specified", "STRAIN OR LINE": "129S2/SvPas"}}, {"GSM723810": {"AGE": "embryo before implantation", "CELL TYPE": "not specified", "STRAIN OR LINE": "129S2/SvPas"}}], "id": 6471, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29281", "summary_wrapped": "Recently, we identified a population of Oct4+Sca-1+Lin-CD45- very small embryonic-like stem-cells (VSELs) in adult tissues. Open...", "pubmed_id": 22023227, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 9, "tags": ["bone", "cell", "chromatin", "genome", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "single-cell-based-genomewide-gene-expression-analy", "geo_id_plat": "E-GEOD-29281_A-AFFY-45", "name": "Single Cell based genomewide gene expression analysis of murine bone-marrow derived Very Small Embryonic-Like Stem Cells (VSELs)", "created": "Nov.11, 2014", "summary": "Recently, we identified a population of Oct4+Sca-1+Lin-CD45- very small embryonic-like stem-cells (VSELs) in adult tissues. Open chromatin structure of pluripotency genes and genomic imprinting-related epigenetic mechanisms maintain pluripotency and quiescence of VSELs, respectively. However, global transcriptome signature of this rare stem-cell population remains elusive. Here, we demonstrate by genomewide gene-expression analysis with a small number of highly purified murine bone-marrow (BM)-derived VSELs, that Oct4+ VSELs i) express a similar, yet nonidentical, transcriptome as embryonic stem-cells (ESCs), ii) up-regulate cell-cycle checkpoint genes, iii) down-regulate genes involved in protein turnover and mitogenic pathways, and iv) highly express Ezh2, a polycomb group protein. Furthermore, as a result of Ezh2 overexpression, VSELs, like ESCs, exhibit bivalently modified nucleosomes (trimethylated H3K27 and H3K4) at promoters of important homeodomain-containing developmental transcription factors, thus preventing their premature transcription. Notably, spontaneous or RNA interference-enforced down-regulation of Ezh2 during VSEL differentiation removes the bivalent-domain (BD) structure, which leads to de-repression of several BD-regulated genes. Therefore, we suggest that VSELs, like other pluripotent stem-cells, maintain their pluripotent state through an Ezh2-dependent BD-mediated epigenetic mechanism. T7-primed cDNA libraries was synthesized using FACS sorted twenty cells. T7-primed libraries were biotin-labeled using the GeneChip 3\u2019 in vitro transcription (IVT) kit (Affymetrix), starting from \u2018\u2018In vitro Transcription to Synthesize Labeled aRNA. Nine Samples (3 VSELs, 3 HSC, and 3 ESC-D3) are analyzed using GeneChip 3\u2019 Mouse Genome 430 2.0 array (Affymetrix), according to the manufacturer\u2019s instructions.", "geo_gse_id": "E-GEOD-29281", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29281/samples/"}