{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6470, "factors": [{"GSM723290": {"BATCH": 1, "GENOTYPE": "Wild type Treg"}}, {"GSM72329": {"BATCH": 1, "GENOTYPE": "Ebi3 \u2013/\u2013 Treg"}}, {"GSM723292": {"BATCH": 1, "GENOTYPE": "Ebi3 \u2013/\u2013 Il10 \u2013/\u2013  Treg"}}, {"GSM723293": {"BATCH": 1, "GENOTYPE": "Il10 \u2013/\u2013  Treg"}}, {"GSM723294": {"BATCH": 2, "GENOTYPE": "Wild type Treg"}}, {"GSM723295": {"BATCH": 2, "GENOTYPE": "Ebi3 \u2013/\u2013 Treg"}}, {"GSM723296": {"BATCH": 2, "GENOTYPE": "Il10 \u2013/\u2013  Treg"}}, {"GSM723297": {"BATCH": 2, "GENOTYPE": "Ebi3 \u2013/\u2013 Il10 \u2013/\u2013  Treg"}}, {"GSM723298": {"BATCH": 3, "GENOTYPE": "Wild type Treg"}}, {"GSM723299": {"BATCH": 3, "GENOTYPE": "Il10 \u2013/\u2013  Treg"}}, {"GSM723300": {"BATCH": 3, "GENOTYPE": "Ebi3 \u2013/\u2013 Treg"}}, {"GSM72330": {"BATCH": 3, "GENOTYPE": "Ebi3 \u2013/\u2013 Il10 \u2013/\u2013  Treg"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Regulatory T cells (Tregs) can suppress a wide variety of cell types, in diverse organ sites and inflammatory conditions.  While Tregs...", "pubmed_id": 22013112, "geo_gse_id": "E-GEOD-29262", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 12, "tags": ["cell", "interleukin", "interleukin-10", "organ"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "functional-plasticity-of-regulatory-t-cell-functio", "geo_id_plat": "E-GEOD-29262_A-AFFY-45", "name": "Functional Plasticity of Regulatory T Cell Function", "created": "Nov.11, 2014", "summary": "Regulatory T cells (Tregs) can suppress a wide variety of cell types, in diverse organ sites and inflammatory conditions.  While Tregs possess multiple suppressive mechanisms, the number required for maximal function is unclear.  Furthermore, whether any inter-relationship orcross-regulatory mechanisms exist that areused to orchestrate and control their utilization is unknown.  Here we assessed the functional capacity of Tregs lacking the ability to secrete both interleukin-10 (IL-10) and IL-35, which individually are required for maximal Treg activity.  Surprisingly, IL-10/IL-35-double deficient Tregswere fully functionalin vitro and in vivo. Loss of IL-10 and IL-35 was compensated for by a concurrent increase in cathepsin E (CTSE) expression, enhanced TRAIL (Tnfsf10)expression and soluble TRAIL release, rendering IL-10/IL-35-double deficient Tregsfunctionally dependent on TRAIL in vitro and in vivo.  Lastly, while C57BL/6 Tregs are IL-10/IL-35-dependent, Balb/c Tregs, which express high levels of CTSE and enhanced TRAIL expression, are TRAIL-dependent.These data reveal that cross-regulatory pathways exist, which control the utilization of suppressive mechanisms,thereby providing Tregfunctional plasticity. Isolate natural Tregs from the different knockout mouse", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29262", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29262/samples/"}