8ArrayExpress Uploader0mousecontrolcontrol0 h0 h24 h24 h48 h48 h8550arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29010Alteration of the PTEN/PI3K pathway is associated with late stage and castrate resistant prostate cancer (CRPC). However, how PTEN loss.../profile/8773/arrayexpressuploader18androgencancerpointprostateprostate cancerviral infectionDec.12, 2014Falsecell-autonomous-role-of-pten-in-regulating-castratE-GEOD-29010_A-AFFY-36Cell autonomous role of PTEN in regulating castration-resistant prostate cancer growthNov.24, 2014Alteration of the PTEN/PI3K pathway is associated with late stage and castrate resistant prostate cancer (CRPC). However, how PTEN loss involves in CRPC development is not clear. Here we show that castration-resistant growth is an intrinsic property of Pten-null prostate cancer (CaP) cells, independent of cancer development stage.PTEN loss suppresses androgen-responsive gene expressions by modulating androgen receptor (AR) transcription factor activity. Conditional deletion of AR in the epithelium promotes the proliferation of Pten-null cancer cells, at least in part, by down-regulating androgen-responsive gene FKBP5 and preventing PHLPP-mediated AKT inhibition. Our findings identify PI3K and AR pathway crosstalk as a mechanism of CRPC development, with potentially important implications for CaP etiology and therapy Mouse embryonic fibroblasts (MEFs) carrying a tet-inducible Pten transgene were generated by retro viral infection and antibiotic selection. Cells were treated with 2 ug/ml doxycycline for 24 or 48 hours in tet-free FBS (5%)/MEF media (n=2). Reference samples were either cells before treatment (n=2). After each time point cells were washed twice with PBS and RNA trizol extracted. WT samples (n =2) were also included as a control.E-GEOD-29010http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29010/samples/