{"platform": 8, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM718745": {"TIME": "control"}}, {"GSM718745": {"TIME": "control"}}, {"GSM718747": {"TIME": "0 h"}}, {"GSM718747": {"TIME": "0 h"}}, {"GSM718749": {"TIME": "24 h"}}, {"GSM718749": {"TIME": "24 h"}}, {"GSM71875": {"TIME": "48 h"}}, {"GSM71875": {"TIME": "48 h"}}], "id": 8550, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29010", "summary_wrapped": "Alteration of the PTEN/PI3K pathway is associated with late stage and castrate resistant prostate cancer (CRPC).  However, how PTEN loss...", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 8, "tags": ["androgen", "cancer", "point", "prostate", "prostate cancer", "viral infection"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "cell-autonomous-role-of-pten-in-regulating-castrat", "geo_id_plat": "E-GEOD-29010_A-AFFY-36", "name": "Cell autonomous role of PTEN in regulating castration-resistant prostate cancer growth", "created": "Nov.24, 2014", "summary": "Alteration of the PTEN/PI3K pathway is associated with late stage and castrate resistant prostate cancer (CRPC).  However, how PTEN loss involves in CRPC development is not clear.  Here we show that castration-resistant growth is an intrinsic property of Pten-null prostate cancer (CaP) cells, independent of cancer development stage.PTEN loss suppresses androgen-responsive gene expressions by modulating androgen receptor (AR) transcription factor activity. Conditional deletion of AR in the epithelium promotes the proliferation of Pten-null cancer cells, at least in part, by down-regulating androgen-responsive gene FKBP5 and preventing PHLPP-mediated AKT inhibition. Our findings identify PI3K and AR pathway crosstalk as a mechanism of CRPC development, with potentially important implications for CaP etiology and therapy Mouse embryonic fibroblasts (MEFs) carrying a tet-inducible Pten transgene were generated by retro viral infection and antibiotic selection.  Cells were treated with 2 ug/ml doxycycline for 24 or 48 hours in tet-free FBS (5%)/MEF media (n=2).  Reference samples were either cells before treatment (n=2).  After each time point cells were washed twice with PBS and RNA trizol extracted.  WT samples (n =2) were also included as a control.", "geo_gse_id": "E-GEOD-29010", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-29010/samples/"}