ArrayExpress Uploader0humanOVCAR-3 parental (progenitor)adherent cultureATCC, Manassas, VAOVCAR-3 parental (progenitor)adherent cultureATCC, Manassas, VAOVCAR-3 parental (progenitor)adherent cultureATCC, Manassas, VAOVCAR-3_derived stem cell-like cellssuspension cultureOvarian Cancer InstituteOVCAR-3_derived stem cell-like cellssuspension cultureOvarian Cancer InstituteOVCAR-3_derived stem cell-like cellssuspension cultureOvarian Cancer Institute4062arrayexpress_sid4Increasing evidence supports the existence of a subpopulation of cancer cells capable of self-renewal and differentiation into diverse...E-GEOD-28799/profile/8773/arrayexpressuploader36cancercellcell phenotypeepithelial celllineovarian cancerstem cellDec.12, 2014Falseisolation-and-characterization-of-stem-like-cellsE-GEOD-28799_A-AFFY-44Isolation and characterization of stem-like cells from a human ovarian cancer cell lineSep.16, 2014Increasing evidence supports the existence of a subpopulation of cancer cells capable of self-renewal and differentiation into diverse cell lineages. These cancer stem-like or cancer initiating cells (CICs) also demonstrate resistance to chemo- and radiotherapy and may function as a primary source of cancer recurrence. We report here on the isolation and in vitro propagation of multicellular ovarian cancer spheroids from a well-established ovarian cancer cell line (OVCAR-3). Cells forming these spheroids display self-renewal potential, the ability to produce differentiated progeny and increased expression of genes previously associated with CICs. The spheroid-derived cells (SDCs) also demonstrate higher invasiveness, migration potential and enhanced resistance to standard anticancer agents relative to progenitor OVCAR-3 cells. SDCs display up-regulation of genes associated with epithelial-to-mesenchymal transitions (EMT), anticancer drug resistance and/or decreased susceptibility to apoptosis, as well as, down-regulation of genes typically associated with the epithelial cell phenotype and pro-apoptotic genes. Dataset includes 3 replicate cultures of parental OVCAR-3 cells and 3 replicate cultures of stem cell-like spheroid-derived cellshttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28799http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28799/samples/