{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM713222": {"CELL LINE": "OVCAR-3 parental (progenitor)", "CULTURE TYPE": "adherent culture", "BIOSOURCEPROVIDER": "ATCC, Manassas, VA"}}, {"GSM713222": {"CELL LINE": "OVCAR-3 parental (progenitor)", "CULTURE TYPE": "adherent culture", "BIOSOURCEPROVIDER": "ATCC, Manassas, VA"}}, {"GSM713222": {"CELL LINE": "OVCAR-3 parental (progenitor)", "CULTURE TYPE": "adherent culture", "BIOSOURCEPROVIDER": "ATCC, Manassas, VA"}}, {"GSM713227": {"CELL LINE": "OVCAR-3_derived stem cell-like cells", "CULTURE TYPE": "suspension culture", "BIOSOURCEPROVIDER": "Ovarian Cancer Institute"}}, {"GSM713227": {"CELL LINE": "OVCAR-3_derived stem cell-like cells", "CULTURE TYPE": "suspension culture", "BIOSOURCEPROVIDER": "Ovarian Cancer Institute"}}, {"GSM713227": {"CELL LINE": "OVCAR-3_derived stem cell-like cells", "CULTURE TYPE": "suspension culture", "BIOSOURCEPROVIDER": "Ovarian Cancer Institute"}}], "id": 4062, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Increasing evidence supports the existence of a subpopulation of cancer cells capable of self-renewal and differentiation into diverse...", "geo_gse_id": "E-GEOD-28799", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 6, "tags": ["cancer", "cell", "cell phenotype", "epithelial cell", "line", "ovarian cancer", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "isolation-and-characterization-of-stem-like-cells", "geo_id_plat": "E-GEOD-28799_A-AFFY-44", "name": "Isolation and characterization of stem-like cells from a human ovarian cancer cell line", "created": "Sep.16, 2014", "summary": "Increasing evidence supports the existence of a subpopulation of cancer cells capable of self-renewal and differentiation into diverse cell lineages. These cancer stem-like or cancer initiating cells (CICs) also demonstrate resistance to chemo- and radiotherapy and may function as a primary source of cancer recurrence. We report here on the isolation and in vitro propagation of multicellular ovarian cancer spheroids from a well-established ovarian cancer cell line (OVCAR-3). Cells forming these spheroids display self-renewal potential, the ability to produce differentiated progeny and increased expression of genes previously associated with CICs. The spheroid-derived cells (SDCs) also demonstrate higher invasiveness, migration potential and enhanced resistance to standard anticancer agents relative to progenitor OVCAR-3 cells. SDCs display up-regulation of genes associated with epithelial-to-mesenchymal transitions (EMT), anticancer drug resistance and/or decreased susceptibility to apoptosis, as well as, down-regulation of genes typically associated with the epithelial cell phenotype and pro-apoptotic genes.  Dataset includes 3 replicate cultures of parental OVCAR-3 cells and 3 replicate cultures of stem cell-like spheroid-derived cells", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28799", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28799/samples/"}