ArrayExpress Uploader0humannormoxianot specifiedhypoxia3 hrshypoxia6 hrshypoxia16 hrsnormoxianot specifiedhypoxia3 hrshypoxia6 hrshypoxia16 hrsnormoxianot specifiedhypoxia3 hrshypoxia6 hrshypoxia16 hrs4047arrayexpress_sid4Hypoxia in adipose tissue is suggested to be involved in the development of a chronic mild inflammation, which in obesity can further...E-GEOD-28603/profile/8773/arrayexpressuploader212adipose tissuecentralgenomeinsulinobesitysyndromeDec.12, 2014Falseexpression-data-from-human-sgbs-adipocytes-under-hE-GEOD-28603_A-AFFY-44Expression data from human SGBS adipocytes under hypoxic conditionsSep.16, 2014Hypoxia in adipose tissue is suggested to be involved in the development of a chronic mild inflammation, which in obesity can further lead to insulin-resistance. The effect of hypoxia on gene expression in adipocytes seems to play a central role in this inflammatory response observed in obesity. However, the global impact of hypoxia on transcriptional changes in human adipocytes is unclear. Therefore, we compared gene expression profiles of human Simpson-Golabi-Behmel syndrome (SGBS) adipocytes under normoxic or hypoxic conditions to detect hypoxia-responsive genes in adipocytes by using whole human genome microarrays. Human SGBS adipocytes were cultured in a hypoxic environment (1% O2) for 3, 6 and 16 hours and the control group was cultured under normoxic conditions (21% O2). Total RNA was prepared from control and treated SGBS cells, in triplicate experiments, and probes were hybridized on ‘Human Genome U133 2.0’ arrays (Affymetrix).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28603http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28603/samples/