{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM707747": {"CATALOG#": "not specified", "STRAIN": "C57BL/6", "ANTIBODY": "not specified", "TREATMENT": "regular diet", "GENOTYPE": "WT"}}, {"GSM707747": {"CATALOG#": "not specified", "STRAIN": "C57BL/6", "ANTIBODY": "not specified", "TREATMENT": "regular diet", "GENOTYPE": "WT"}}, {"GSM707749": {"CATALOG#": "not specified", "STRAIN": "C57BL/6", "ANTIBODY": "not specified", "TREATMENT": "high fat diet", "GENOTYPE": "WT"}}, {"GSM707749": {"CATALOG#": "not specified", "STRAIN": "C57BL/6", "ANTIBODY": "not specified", "TREATMENT": "high fat diet", "GENOTYPE": "WT"}}, {"GSM70775": {"CATALOG#": "not specified", "STRAIN": "mixed strains of C57BL/6 and NIH Black Swiss females (Taconic)", "ANTIBODY": "not specified", "TREATMENT": "regular diet", "GENOTYPE": "Smad3-/-"}}, {"GSM70775": {"CATALOG#": "not specified", "STRAIN": "mixed strains of C57BL/6 and NIH Black Swiss females (Taconic)", "ANTIBODY": "not specified", "TREATMENT": "regular diet", "GENOTYPE": "Smad3-/-"}}, {"GSM707753": {"CATALOG#": "not specified", "STRAIN": "mixed strains of C57BL/6 and NIH Black Swiss females (Taconic)", "ANTIBODY": "not specified", "TREATMENT": "high fat diet", "GENOTYPE": "Smad3-/-"}}, {"GSM707753": {"CATALOG#": "not specified", "STRAIN": "mixed strains of C57BL/6 and NIH Black Swiss females (Taconic)", "ANTIBODY": "not specified", "TREATMENT": "high fat diet", "GENOTYPE": "Smad3-/-"}}, {"GSM707755": {"CATALOG#": "13C4", "STRAIN": "C57J/B6", "ANTIBODY": "IgG", "TREATMENT": "diet induced obese", "GENOTYPE": "not specified"}}, {"GSM707755": {"CATALOG#": "13C4", "STRAIN": "C57J/B6", "ANTIBODY": "IgG", "TREATMENT": "diet induced obese", "GENOTYPE": "not specified"}}, {"GSM707757": {"CATALOG#": "1D11", "STRAIN": "C57J/B6", "ANTIBODY": "TGF-beta", "TREATMENT": "diet induced obese", "GENOTYPE": "not specified"}}, {"GSM707757": {"CATALOG#": "1D11", "STRAIN": "C57J/B6", "ANTIBODY": "TGF-beta", "TREATMENT": "diet induced obese", "GENOTYPE": "not specified"}}], "id": 6444, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Imbalances in glucose and energy homeostasis are at the core of the worldwide epidemic of obesity and diabetes. Here, we illustrate an...", "pubmed_id": 21723505, "geo_gse_id": "E-GEOD-28598", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 5, "sample_count": 12, "tags": ["adipose tissue", "basal", "glucose", "muscle", "obesity", "white adipose tissue"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-28598_A-AFFY-45", "slug": "protection-from-obesity-and-diabetes-by-blockade-o", "geo_gds_id": "", "name": "Protection from obesity and diabetes by blockade of TGF-beta/Smad3 signaling", "created": "Nov.11, 2014", "summary": "Imbalances in glucose and energy homeostasis are at the core of the worldwide epidemic of obesity and diabetes. Here, we illustrate an important role of the TGF-beta/Smad3 signaling pathway in regulating glucose and energy homeostasis. Smad3 deficient mice are protected from diet-induced obesity and diabetes. Interestingly, the metabolic protection is accompanied by Smad3-/- white adipose tissue acquiring the bioenergetic and gene expression profile of brown fat/skeletal muscle. Smad3-/- adipocytes demonstrate a marked increase in mitochondrial biogenesis, with a corresponding increase in basal respiration, and Smad3 acts as a repressor of PGC-alpha1 expression. We observe significant correlation between TGF-beta1 levels and adiposity in rodents and humans. Further, systemic blockade of TGF-beta1 signaling protects mice from obesity, diabetes and hepatic steatosis. Together, these results demonstrate that TGF-beta signaling regulates glucose tolerance and energy homeostasis and suggest that modulation of TGF-beta1 activity might be an effective treatment strategy for obesity and diabetes. Smad3-/- and WT mice were fed with regular diet (RD) and high fat diet (HFD), and diet induced obese (DIO) mice were treated with IgG and anti-TGF-b1 antibody", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28598", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28598/samples/"}