ArrayExpress Uploaderarrayexpress_sid4046controlnonenot specifiedcontrolTGF-beta1.5 hrscontrolTGF-beta24 hrsHNF4anonenot specifiedHNF4aTGF-beta1.5 hrsHNF4aTGF-beta24 hrs04Smad2/3 are transcription factors that engage in TGF-beta-induced transcription. We determined and analyzed HepG2 and Hep3B-specific...21646355/profile/8773/arrayexpressuploader36Dec.12, 2014FalseE-GEOD-28590_A-AFFY-44expression-data-of-the-human-hepatoblastoma-cell-lhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28590Expression data of the human hepatoblastoma cell line HepG2 treated with TGF-betaSep.16, 2014Smad2/3 are transcription factors that engage in TGF-beta-induced transcription. We determined and analyzed HepG2 and Hep3B-specific Smad2/3 binding sites by ChIP-chip. We used expression microarrays to compare the Smad2/3 and HNF4alpha binding sites identified by ChIP-chip or ChIP-seq, respectively, to TGF-beta-induced gene expressions. HepG2 cells were transfected with control or HNF4A siRNAs and treated with 3 ng/ml TGF-beta for 0, 1.5 and 24 h (6 samples in total, no replicates). Total RNA was extracted and expression microarray analysis was performed as described in the protocols.E-GEOD-28590humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28590/samples/