{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM703137 1": {}}, {"GSM703138 1": {}}, {"GSM703139 1": {}}, {"GSM703140 1": {}}, {"GSM703141 1": {}}, {"GSM703142 1": {}}, {"GSM703143 1": {}}, {"GSM703144 1": {}}, {"GSM703145 1": {}}, {"GSM703146 1": {}}, {"GSM703147 1": {}}], "id": 4035, "pop_total": 0, "platform": 4, "summary_wrapped": "TGF\u03b2 is known to be a potent inducer of EMT, a process involved in tumor invasion. TIF1\u03b3 has been reported to participate to TGF\u03b2...", "geo_gse_id": "E-GEOD-28448", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 11, "tags": ["cell", "epithelial cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "antagonistic-regulation-of-emt-by-tif1-and-smad4-i", "geo_id_plat": "E-GEOD-28448_A-AFFY-44", "name": "Antagonistic regulation of EMT by TIF1\u03b3 and Smad4 in mammary epithelial cells", "created": "Sep.16, 2014", "summary": "TGF\u03b2 is known to be a potent inducer of EMT, a process involved in tumor invasion. TIF1\u03b3 has been reported to participate to TGF\u03b2 signaling. In order to understand the role of TIF1\u03b3 in TGF\u03b2 signaling and its requirement for EMT, we analyzed the TGF\u03b21 response of human mammary epithelial cell lines. A strong EMT increase was observed in TIF1\u03b3-silenced cells after TGF\u03b21 treatment, whereas Smad4 inactivation completely blocked this process. In support of these observations, microarray data show that the functions of several TIF1\u03b3 target genes can be linked to EMT. As a negative regulator of Smad4, TIF1\u03b3 could be critical for the regulation of TGF\u03b2 signaling. This work highlights the molecular relationship between TIF1\u03b3 and Smad4 in TGF\u03b21 signaling and EMT. Total mRNA extractions were performed for 11 samples from transfected HMEC-TR. Replicates are rimo1, 6; rimo3, 9; rimo2, 7; rimo 4, 10 and rimo 5, 11. Rimo 8 is a single experiment. All RNA extractions were obtained from two independent cell cultures excepted for rimo8. Rimo 1, 6 are replicate for ctrl-; Rimo 3, 9 are replicate for ctrl+; Rimo 2, 7 are replicate for SiTIF-; Rimo 4, 10 are replicate for SiTIF+;  Rimo8 is SiSmad4-;  and Rimo5, 11 are replicate for SiSmad4+. ctrl means that HMEC-TR were transfected with an SiRNA scramble. \"siSmad4\" means  that HMEC-TR were transfected with an SiRNA anti Smad4.  \"siTIF\" means  that HMEC-TR were transfected with an SiRNA anti TIF1\u03b3.   \"-\" means that cells were grown without TGF\u03b2. \"+\" means that cells were treated with 5 ng/ml TGF\u03b21 for 24h.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28448", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28448/samples/"}