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Home › Dataset Library › Effects of resistance exercise on the transcriptome in MHC I and MHC IIa muscle fibers of young and old women

Dataset: Effects of resistance exercise on the transcriptome in MHC I and MHC IIa muscle fibers of young and old women

Global microarray (HG U133 Plus 2.0) was used for the first time to investigate the effects of resistance exercise on the transcriptome...

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Global microarray (HG U133 Plus 2.0) was used for the first time to investigate the effects of resistance exercise on the transcriptome in slow-twitch myosin heavy chain (MHC) I and fast-twitch MHC IIa muscle fibers of young and old women. Vastus lateralis muscle biopsies were obtained pre and 4hrs post resistance exercise in the beginning (untrained state) and at the end (trained state) of a 12 wk progressive resistance training program. A total of 14 females were included in this investigation. The participants included 8 young (23±2y) and 6 old (85±1y) females. All subjects participated in 12 wks of progressive resistance training consisting of bilateral knee extensions with 3x10 reps at 70% of 1-RM, and 3d/wk for a total of 36 training sessions. Vastus lateralis biopsies were obtained in conjunction with the 1st and 36th (last) training session and included a basal biopsy and another biopsy 4hrs post the resistance exercise session. From each biopsy sample, we isolated individual muscle fibers. After myosin isoform identification of isolated fibers (SDS-PAGE), RNA extraction of 20 MHC I and 20 MHC IIa muscle fibers per biopsy sample followed. Thus, each resulting sample contained total RNA from 20 muscle fibers of identical fiber type (MHC I or MHC IIa). A total of 70 samples were analyzed on separate microarray chips, and samples were not pooled between subjects. The study design allowed us to examine the acute effects of resistance exercise on the transcriptome in MHC I and MHC IIa muscle fibers in the untrained and trained state.

Species:
human

Samples:
70

Source:
E-GEOD-28392

Updated:
Dec.12, 2014

Registered:
Sep.16, 2014


Factors: (via ArrayExpress)
Sample FIBER TYPE AGE TRAINING STATE TIME POINT SUBJECT
GSM701766 slow-twitch (MHC I) Young (23±2y) Untrained basal young female 2 (YF_2)
GSM701767 slow-twitch (MHC I) Young (23±2y) Untrained basal young female 3 (YF_3)
GSM701768 slow-twitch (MHC I) Young (23±2y) Untrained basal young female 4 (YF_4)
GSM701769 slow-twitch (MHC I) Young (23±2y) Untrained basal young female 6 (YF_6)
GSM701770 slow-twitch (MHC I) Young (23±2y) Untrained basal young female 8 (YF_8)
GSM70177 slow-twitch (MHC I) Young (23±2y) Untrained 4hrs post exercise young female 1 (YF_1)
GSM701772 slow-twitch (MHC I) Young (23±2y) Untrained 4hrs post exercise young female 2 (YF_2)
GSM701773 slow-twitch (MHC I) Young (23±2y) Untrained 4hrs post exercise young female 6 (YF_6)
GSM701774 slow-twitch (MHC I) Young (23±2y) Untrained 4hrs post exercise young female 7 (YF_7)
GSM701775 slow-twitch (MHC I) Young (23±2y) Untrained 4hrs post exercise young female 8 (YF_8)
GSM701776 slow-twitch (MHC I) Young (23±2y) Trained basal young female 1 (YF_1)
GSM701777 slow-twitch (MHC I) Young (23±2y) Trained basal young female 2 (YF_2)
GSM701778 slow-twitch (MHC I) Young (23±2y) Trained basal young female 3 (YF_3)
GSM701779 slow-twitch (MHC I) Young (23±2y) Trained basal young female 4 (YF_4)
GSM701780 slow-twitch (MHC I) Young (23±2y) Trained basal young female 7 (YF_7)
GSM70178 slow-twitch (MHC I) Young (23±2y) Trained 4hrs post exercise young female 1 (YF_1)
GSM701782 slow-twitch (MHC I) Young (23±2y) Trained 4hrs post exercise young female 2 (YF_2)
GSM701783 slow-twitch (MHC I) Young (23±2y) Trained 4hrs post exercise young female 3 (YF_3)
GSM701784 slow-twitch (MHC I) Young (23±2y) Trained 4hrs post exercise young female 4 (YF_4)
GSM701785 slow-twitch (MHC I) Young (23±2y) Trained 4hrs post exercise young female 6 (YF_6)
GSM701786 slow-twitch (MHC I) Young (23±2y) Trained 4hrs post exercise young female 7 (YF_7)
GSM701787 slow-twitch (MHC I) Old (85±1y) Untrained basal old female 11 (OF_11)
GSM701788 slow-twitch (MHC I) Old (85±1y) Untrained basal old female 12 (OF_12)
GSM701789 slow-twitch (MHC I) Old (85±1y) Untrained basal old female 13 (OF_13)
GSM701790 slow-twitch (MHC I) Old (85±1y) Untrained basal old female 14 (OF_14)
GSM70179 slow-twitch (MHC I) Old (85±1y) Untrained 4hrs post exercise old female 9 (OF_9)
GSM701792 slow-twitch (MHC I) Old (85±1y) Untrained 4hrs post exercise old female 11 (OF_11)
GSM701793 slow-twitch (MHC I) Old (85±1y) Untrained 4hrs post exercise old female 13 (OF_13)
GSM701794 slow-twitch (MHC I) Old (85±1y) Trained basal old female 10 (OF_10)
GSM701795 slow-twitch (MHC I) Old (85±1y) Trained basal old female 11 (OF_11)
GSM701796 slow-twitch (MHC I) Old (85±1y) Trained basal old female 12 (OF_12)
GSM701797 slow-twitch (MHC I) Old (85±1y) Trained basal old female 13 (OF_13)
GSM701798 slow-twitch (MHC I) Old (85±1y) Trained 4hrs post exercise old female 9 (OF_9)
GSM701799 slow-twitch (MHC I) Old (85±1y) Trained 4hrs post exercise old female 10 (OF_10)
GSM701800 slow-twitch (MHC I) Old (85±1y) Trained 4hrs post exercise old female 11 (OF_11)
GSM70180 slow-twitch (MHC I) Old (85±1y) Trained 4hrs post exercise old female 12 (OF_12)
GSM701802 slow-twitch (MHC I) Old (85±1y) Trained 4hrs post exercise old female 13 (OF_13)
GSM701803 fast-twitch (MHC IIa) Young (23±2y) Untrained basal young female 1 (YF_1)
GSM701804 fast-twitch (MHC IIa) Young (23±2y) Untrained basal young female 2 (YF_2)
GSM701805 fast-twitch (MHC IIa) Young (23±2y) Untrained basal young female 3 (YF_3)
GSM701806 fast-twitch (MHC IIa) Young (23±2y) Untrained basal young female 6 (YF_6)
GSM701807 fast-twitch (MHC IIa) Young (23±2y) Untrained 4hrs post exercise young female 1 (YF_1)
GSM701808 fast-twitch (MHC IIa) Young (23±2y) Untrained 4hrs post exercise young female 2 (YF_2)
GSM701809 fast-twitch (MHC IIa) Young (23±2y) Untrained 4hrs post exercise young female 3 (YF_3)
GSM701810 fast-twitch (MHC IIa) Young (23±2y) Untrained 4hrs post exercise young female 7 (YF_7)
GSM7018 fast-twitch (MHC IIa) Young (23±2y) Trained basal young female 1 (YF_1)
GSM701812 fast-twitch (MHC IIa) Young (23±2y) Trained basal young female 2 (YF_2)
GSM701813 fast-twitch (MHC IIa) Young (23±2y) Trained basal young female 3 (YF_3)
GSM701814 fast-twitch (MHC IIa) Young (23±2y) Trained basal young female 5 (YF_5)
GSM701815 fast-twitch (MHC IIa) Young (23±2y) Trained basal young female 6 (YF_6)
GSM701816 fast-twitch (MHC IIa) Young (23±2y) Trained basal young female 8 (YF_8)
GSM701817 fast-twitch (MHC IIa) Young (23±2y) Trained 4hrs post exercise young female 1 (YF_1)
GSM701818 fast-twitch (MHC IIa) Young (23±2y) Trained 4hrs post exercise young female 2 (YF_2)
GSM701819 fast-twitch (MHC IIa) Young (23±2y) Trained 4hrs post exercise young female 3 (YF_3)
GSM701820 fast-twitch (MHC IIa) Young (23±2y) Trained 4hrs post exercise young female 4 (YF_4)
GSM70182 fast-twitch (MHC IIa) Young (23±2y) Trained 4hrs post exercise young female 5 (YF_5)
GSM701822 fast-twitch (MHC IIa) Young (23±2y) Trained 4hrs post exercise young female 6 (YF_6)
GSM701823 fast-twitch (MHC IIa) Old (85±1y) Untrained basal old female 10 (OF_10)
GSM701824 fast-twitch (MHC IIa) Old (85±1y) Untrained basal old female 11 (OF_11)
GSM701825 fast-twitch (MHC IIa) Old (85±1y) Untrained 4hrs post exercise old female 9 (OF_9)
GSM701826 fast-twitch (MHC IIa) Old (85±1y) Untrained 4hrs post exercise old female 11 (OF_11)
GSM701827 fast-twitch (MHC IIa) Old (85±1y) Untrained 4hrs post exercise old female 13 (OF_13)
GSM701828 fast-twitch (MHC IIa) Old (85±1y) Trained basal old female 10 (OF_10)
GSM701829 fast-twitch (MHC IIa) Old (85±1y) Trained basal old female 11 (OF_11)
GSM701830 fast-twitch (MHC IIa) Old (85±1y) Trained basal old female 13 (OF_13)
GSM70183 fast-twitch (MHC IIa) Old (85±1y) Trained basal old female 14 (OF_14)
GSM701832 fast-twitch (MHC IIa) Old (85±1y) Trained 4hrs post exercise old female 9 (OF_9)
GSM701833 fast-twitch (MHC IIa) Old (85±1y) Trained 4hrs post exercise old female 10 (OF_10)
GSM701834 fast-twitch (MHC IIa) Old (85±1y) Trained 4hrs post exercise old female 11 (OF_11)
GSM701835 fast-twitch (MHC IIa) Old (85±1y) Trained 4hrs post exercise old female 13 (OF_13)

Tags

  • basal
  • fiber
  • knee
  • muscle
  • myosin
  • myosin heavy chain
  • vastus lateralis

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