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Home › Dataset Library › Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells

Dataset: Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells

Although practiced clinically for more than 40 years, the use of hematopoietic stem cell (HSC) transplants remains limited by the ability...

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Although practiced clinically for more than 40 years, the use of hematopoietic stem cell (HSC) transplants remains limited by the ability to expand these cells ex vivo. An unbiased screen with primary human HSCs identified a purine derivative, StemRegenin 1 (SR1), that promotes the ex vivo expansion of CD34+ cells. Culture of HSCs with SR1 led to a 50-fold increase in cells expressing CD34 and a 17-fold increase in cells that retain the ability to engraft immunodeficient mice. Mechanistic studies show that SR1 acts by antagonizing the aryl hydrocarbon receptor (AHR). The identification of SR1 and AHR modulation as a means to induce ex vivo HSC expansion should facilitate the clinical use of HSC therapy. LGC006, a less potent SR1 analog, was also examined. KEYWORDS: two compounds, multiple doses, one time point two compounds, multiple doses, one time point

Species:
human

Samples:
8

Source:
E-GEOD-28359

PubMed:
20688981

Updated:
Dec.12, 2014

Registered:
Sep.16, 2014


Factors: (via ArrayExpress)
Sample AGENT DOSE
GSM701153 SR1 0nM
GSM701154 SR1 30nM
GSM701155 SR1 100nM
GSM701156 SR1 300nM
GSM701157 SR1 1000nM
GSM701158 LGC006 30nM
GSM701159 LGC006 300nM
GSM701160 LGC006 1000nM

Tags

  • cell
  • point
  • purine
  • stem cell

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