{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM700062": {"CULTURE CONDITION": "not specified", "CHIP ANTIBODY VENDOR": "not specified", "BATCH NUMBER": "not specified", "CULTURE CONDITIONS": "Th1", "CHIP ANTIBODY": "not specified", "CELL TYPE": "CD4 T cell"}}, {"GSM700063": {"CULTURE CONDITION": "not specified", "CHIP ANTIBODY VENDOR": "not specified", "BATCH NUMBER": "not specified", "CULTURE CONDITIONS": "Th2", "CHIP ANTIBODY": "not specified", "CELL TYPE": "CD4 T cell"}}], "id": 6427, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28292", "summary_wrapped": "Differentiation of naive CD4 T cells into type 2 helper (Th2) cells is accompanied by chromatin remodeling and increased expression of a...", "pubmed_id": 21536806, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 6, "sample_count": 2, "tags": ["cell", "chromatin", "cytokine", "genome", "histone"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "genome-wide-analysis-reveals-unique-regulation-of", "geo_id_plat": "E-GEOD-28292_A-AFFY-45", "name": "Genome-wide analysis reveals unique regulation of transcription of Th2-specific genes by GATA3", "created": "Nov.11, 2014", "summary": "Differentiation of naive CD4 T cells into type 2 helper (Th2) cells is accompanied by chromatin remodeling and increased expression of a set of Th2-specific genes including those encoding Th2 cytokines.  IL-4-mediated STAT6 activation induces high levels of transcription of GATA3, a master regulator of Th2 cell differentiation, and enforced expression of GATA3 induces Th2 cytokine expression.  However, it remains unclear whether the expression of other Th2-specific genes is induced directly by GATA3.  A genome-wide unbiased ChIP-seq analysis revealed that GATA3 bound to 1,279 genes selectively in Th2 cells, and 101 genes in both Th1 and Th2 cells.  Simultaneously, we identified 26 highly Th2-specific STAT6-dependent inducible genes by a DNA microarray analysis-based three-step selection processes, and among them 17 genes showed GATA3 binding.  We assessed dependency on GATA3 for the transcription of these 26 Th2-specific genes, and 10 genes showed increased transcription in a GATA3-dependent manner while 16 genes showed no significant responses.  The transcription of the 16 GATA3-nonresponding genes was clearly increased by the introduction of an active form of STAT6, STAT6VT.  Therefore, although GATA3 has been recognized as a master regulator of Th2 cell differentiation, many Th2-specific genes are not regulated by GATA3 itself but in collaboration with STAT6. Th1 and Th2  subsets are profiled for mRNA expression Examination of GATA3-binding and 3 different histone modifications in Th1 and Th2 cells.", "geo_gse_id": "E-GEOD-28292", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28292/samples/"}