ArrayExpress Uploader0humanHIV-HIV-HIV-HIV+HIV+HIV+4015arrayexpress_sid4We evaluated longitudinal changes in viral replication and emergence of viral variants in the context of T cell homeostasis and gene...E-GEOD-28177/profile/8773/arrayexpressuploader16cellguthiv infectionlongitudinalperipheralDec.12, 2014Falseexpression-data-from-haart-interruption-in-hiv-patE-GEOD-28177_A-AFFY-44Expression data from HAART interruption in HIV patientsSep.16, 2014We evaluated longitudinal changes in viral replication and emergence of viral variants in the context of T cell homeostasis and gene expression in GALT of three HIV-positive patients who initiated HAART during primary HIV infection but opted to interrupt therapy thereafter. Longitudinal viral sequence analysis revealed that a stable proviral reservoir was established in GALT during primary HIV infection that persisted through early HAART and post-therapy interruption. Proviral variants in GALT and peripheral blood mononuclear cells (PBMCs) displayed low levels of genomic diversity at all times. A rapid increase in viral loads with a modest decline of CD4 T cells in peripheral blood was observed, while gut mucosal CD4 T cell loss was severe following HAART interruption. This was accompanied by increased mucosal gene expression regulating interferon (IFN)-mediated antiviral responses and immune activation, a profile similar to those found in HAART-naive HIV-infected patients. Gut mucosal responses to HAART interruption were evaluated with Affymetrix arrayshttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28177http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28177/samples/