ArrayExpress Uploader04006IL-4 treatment for 0hnot specifiednot specifiedIL-4 treatment for 1hnot specifiednot specifiedIL-4 treatment for 2hnot specifiednot specifiedIL-4 treatment for 4hnot specifiednot specifiedIL-4 treatment for 8hnot specifiednot specifiedIL-4 treatment for 16hnot specifiednot specifiedIL-4 treatment for 0hsi-control #1not specifiedIL-4 treatment for 4hsi-control #1not specifiedIL-4 treatment for 4hsi-STAT6 Oligo1not specifiedIL-4 treatment for 4hsi-STAT6 Oligo2not specifiednot specifiedAd-CtrlControl-Adenovirus for 48hnot specifiedAd-CA-STAT6 #1Constitutive Active STAT6 Adenovirus for 48h #1not specifiedAd-CA-STAT6 #2Constitutive Active STAT6 Adenovirus for 48h #2arrayexpress_sid4Endothelial cell activation and dysfunction underlie many vascular disorders, including atherosclerosis and inflammation. Here, we show...21464207/profile/8773/arrayexpressuploader313atherosclerosiscellcell adhesion moleculechromatindeepdiseaseendothelial cellendotheliumgenomeinterleukinmonocytevascular diseaseDec.12, 2014FalseE-GEOD-28117_A-AFFY-44il-4-mediated-gene-expression-profiles-in-vascularhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28117IL-4 mediated gene expression profiles in vascular endothelial cellsSep.15, 2014Endothelial cell activation and dysfunction underlie many vascular disorders, including atherosclerosis and inflammation. Here, we show that interleukin (IL)-4 markedly induced vascular cell adhesion molecule (VCAM)-1, both in cultured endothelial cells and in the intact endothelium in mice. Combined treatment with IL-4 and tumor necrosis factor (TNF)- alpha resulted in further, sustained induction of VCAM-1 expression. IL-4-mediated induction of VCAM-1 and secondary monocyte adhesion was predominantly regulated by the transcription factor, STAT6. Genome-wide survey of IL-4-mediated STAT6 binding from sequential chromatin-immunoprecipitation with deep-sequencing (ChIP-seq) in endothelial cells revealed regions of transient and sustained transcription factor binding. By combining DNA microarrays and ChIP-seq at the same time points, the majority of IL-4-responsive genes were shown to be STAT6-dependent and associated with direct STAT6 binding to their promoter. IL-4-mediated stable binding of STAT6 led to sustained target gene expression. Moreover, our strategy led to the identification of a novel functionally important STAT6 binding site within -16 kb upstream of the VCAM-1 gene. Taken together, these findings support a critical role for STAT6 in mediating IL-4 signal transduction in endothelial cells. Identification of a novel IL-4-mediated VCAM-1 enhancer may provide a foundation for targeted therapy in vascular disease (ChIP-seq data not submitted to GEO). Total 13 samples were derived from [1] HUVEC treated in the absence (0h) or presence of IL-4 (1,2,4,8, and 16h) to determine IL-4 regulated gene in endotherial cells, [2] control-siRNA or si-STAT6 transfected HUVEC cells treated in the absence or presenceof IL-4 [3] Ad-control or Ad-CA-STAT6 transfected HUVEC cells for the identification of STAT-6 dependent genes in endothelial cells.E-GEOD-28117humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28117/samples/