{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 4006, "factors": [{"GSM696436": {"STIMULATION": "IL-4 treatment for 0h", "TREATMENT": "not specified", "INFECTION": "not specified"}}, {"GSM699979": {"STIMULATION": "IL-4 treatment for 1h", "TREATMENT": "not specified", "INFECTION": "not specified"}}, {"GSM699980": {"STIMULATION": "IL-4 treatment for 2h", "TREATMENT": "not specified", "INFECTION": "not specified"}}, {"GSM69998": {"STIMULATION": "IL-4 treatment for 4h", "TREATMENT": "not specified", "INFECTION": "not specified"}}, {"GSM699982": {"STIMULATION": "IL-4 treatment for 8h", "TREATMENT": "not specified", "INFECTION": "not specified"}}, {"GSM699983": {"STIMULATION": "IL-4 treatment for 16h", "TREATMENT": "not specified", "INFECTION": "not specified"}}, {"GSM703089": {"STIMULATION": "IL-4 treatment for 0h", "TREATMENT": "si-control #1", "INFECTION": "not specified"}}, {"GSM707972": {"STIMULATION": "IL-4 treatment for 4h", "TREATMENT": "si-control #1", "INFECTION": "not specified"}}, {"GSM707973": {"STIMULATION": "IL-4 treatment for 4h", "TREATMENT": "si-STAT6 Oligo1", "INFECTION": "not specified"}}, {"GSM707974": {"STIMULATION": "IL-4 treatment for 4h", "TREATMENT": "si-STAT6 Oligo2", "INFECTION": "not specified"}}, {"GSM707975": {"STIMULATION": "not specified", "TREATMENT": "Ad-Ctrl", "INFECTION": "Control-Adenovirus for 48h"}}, {"GSM707976": {"STIMULATION": "not specified", "TREATMENT": "Ad-CA-STAT6 #1", "INFECTION": "Constitutive Active STAT6 Adenovirus for 48h #1"}}, {"GSM707977": {"STIMULATION": "not specified", "TREATMENT": "Ad-CA-STAT6 #2", "INFECTION": "Constitutive Active STAT6 Adenovirus for 48h #2"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Endothelial cell activation and dysfunction underlie many vascular disorders, including atherosclerosis and inflammation. Here, we show...", "pubmed_id": 21464207, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 13, "tags": ["atherosclerosis", "cell", "cell adhesion molecule", "chromatin", "deep", "disease", "endothelial cell", "endothelium", "genome", "interleukin", "monocyte", "vascular disease"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-28117_A-AFFY-44", "slug": "il-4-mediated-gene-expression-profiles-in-vascular", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28117", "geo_gds_id": "", "name": "IL-4 mediated gene expression profiles in vascular endothelial cells", "created": "Sep.15, 2014", "summary": "Endothelial cell activation and dysfunction underlie many vascular disorders, including atherosclerosis and inflammation. Here, we show that interleukin (IL)-4 markedly induced vascular cell adhesion molecule (VCAM)-1, both in cultured endothelial cells and in the intact endothelium in mice. Combined treatment with IL-4 and tumor necrosis factor (TNF)- alpha resulted in further, sustained induction of VCAM-1 expression. IL-4-mediated induction of VCAM-1 and secondary monocyte adhesion was predominantly regulated by the transcription factor, STAT6. Genome-wide survey of IL-4-mediated STAT6 binding from sequential chromatin-immunoprecipitation with deep-sequencing (ChIP-seq) in endothelial cells revealed regions of transient and sustained transcription factor binding. By combining DNA microarrays and ChIP-seq at the same time points, the majority of IL-4-responsive genes were shown to be STAT6-dependent and associated with direct STAT6 binding to their promoter. IL-4-mediated stable binding of STAT6 led to sustained target gene expression. Moreover, our strategy led to the identification of a novel functionally important STAT6 binding site within -16 kb upstream of the VCAM-1 gene. Taken together, these findings support a critical role for STAT6 in mediating IL-4 signal transduction in endothelial cells. Identification of a novel IL-4-mediated VCAM-1 enhancer may provide a foundation for targeted therapy in vascular disease (ChIP-seq data not submitted to GEO). Total 13 samples were derived from [1] HUVEC treated in the absence (0h) or presence of IL-4 (1,2,4,8, and 16h) to determine IL-4 regulated gene in endotherial cells, [2] control-siRNA or si-STAT6 transfected HUVEC cells treated in the absence or presenceof IL-4 [3] Ad-control or Ad-CA-STAT6 transfected HUVEC cells for the identification of STAT-6 dependent genes in endothelial cells.", "geo_gse_id": "E-GEOD-28117", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-28117/samples/"}