ArrayExpress Uploaderarrayexpress_sidmousep53-/-vehiclep53-/-vehiclep53-/-rapamycinp53-/-rapamycinp53-/-, Tsc2-/-vehiclep53-/-, Tsc2-/-vehiclep53-/-, Tsc2-/-rapamycinp53-/-, Tsc2-/-rapamycin827007Mammalian target of rapamycin (mTOR) complex 1 (mTORC1) is a critical regulator of cell growth by integrating multiple signals...21804531E-GEOD-27982/profile/8773/arrayexpressuploader28cancercellembryoserumDec.12, 2014FalseE-GEOD-27982_A-AFFY-23transcription-profiling-by-array-of-mouse-immortalTranscription profiling by array of mouse immortalized embryonic fibroblasts to identify genes regulated by mTORC1Nov.23, 2014Mammalian target of rapamycin (mTOR) complex 1 (mTORC1) is a critical regulator of cell growth by integrating multiple signals (nutrients, growth factors, energy and stress) and is frequently deregulated in many types of cancer. We used a robust experimental paradigm involving the combination of two interventions, one genetic and one pharmacologic to identify genes regulated transcriptionally by mTORC1. In Tsc2+/+, but not Tsc2-/- immortalized mouse embryo fibroblasts (MEFs), serum deprivation downregulates mTORC1 activity. In Tsc2-/- cells, abnormal mTORC1 activity can be downregulated by treatment with rapamycin (sirolimus). By contrast, rapamycin has little effect on mTORC1 in Tsc2+/+ cells in which mTORC1 is already inhibited by low serum. Thus, under serum deprived conditions, mTORC1 activity is low in Tsc2+/+ cells (untreated or rapamycin treated), high in Tsc2-/- cells, but lowered by rapamycin; a pattern referred to as a “low/low/high/low” or “LLHL”, which allowed the identification of genes regulated by mTORC1 by performing the appropriate comparisonshttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27982http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27982/samples/