{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM692432": {"GENOTYPE": "p53-/-", "COMPOUND": "vehicle"}}, {"GSM692432": {"GENOTYPE": "p53-/-", "COMPOUND": "vehicle"}}, {"GSM692434": {"GENOTYPE": "p53-/-", "COMPOUND": "rapamycin"}}, {"GSM692434": {"GENOTYPE": "p53-/-", "COMPOUND": "rapamycin"}}, {"GSM692436": {"GENOTYPE": "p53-/-, Tsc2-/-", "COMPOUND": "vehicle"}}, {"GSM692436": {"GENOTYPE": "p53-/-, Tsc2-/-", "COMPOUND": "vehicle"}}, {"GSM692438": {"GENOTYPE": "p53-/-, Tsc2-/-", "COMPOUND": "rapamycin"}}, {"GSM692438": {"GENOTYPE": "p53-/-, Tsc2-/-", "COMPOUND": "rapamycin"}}], "id": 8270, "pop_total": 0, "platform": 7, "summary_wrapped": "Mammalian target of rapamycin (mTOR) complex 1 (mTORC1) is a critical regulator of cell growth by integrating multiple signals...", "pubmed_id": 21804531, "geo_gse_id": "E-GEOD-27982", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 8, "tags": ["cancer", "cell", "embryo", "serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-27982_A-AFFY-23", "slug": "transcription-profiling-by-array-of-mouse-immortal", "geo_gds_id": "", "name": "Transcription profiling by array of mouse immortalized embryonic fibroblasts to identify genes regulated by mTORC1", "created": "Nov.23, 2014", "summary": "Mammalian target of rapamycin (mTOR) complex 1 (mTORC1) is a critical regulator of cell growth by integrating multiple signals (nutrients, growth factors, energy and stress) and is frequently deregulated in many types of cancer. We used a robust experimental paradigm involving the combination of two interventions, one genetic and one pharmacologic to identify genes regulated transcriptionally by mTORC1. In Tsc2+/+, but not Tsc2-/- immortalized mouse embryo fibroblasts (MEFs), serum deprivation downregulates mTORC1 activity. In Tsc2-/- cells, abnormal mTORC1 activity can be downregulated by treatment with rapamycin (sirolimus). By contrast, rapamycin has little effect on mTORC1 in Tsc2+/+ cells in which mTORC1 is already inhibited by low serum. Thus, under serum deprived conditions, mTORC1 activity is low in Tsc2+/+ cells (untreated or rapamycin treated), high in Tsc2-/- cells, but lowered by rapamycin; a pattern referred to as a \u201clow/low/high/low\u201d or \u201cLLHL\u201d, which allowed the identification of genes regulated by mTORC1 by performing the appropriate comparisons", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27982", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27982/samples/"}