ArrayExpress Uploaderarrayexpress_sidratsham shocksham shocksham shocksham shockT/HS with resuscitation with IL6T/HS with resuscitation with PBST/HS with resuscitation with PBST/HS with resuscitation with IL6T/HS with resuscitation with PBST/HS with resuscitation with PBST/HS with resuscitation with IL6GQ-ODN pre-treatment followed by T/HS with resuscitation with IL6GQ-ODN pre-treatment followed by T/HS with resuscitation with IL6GQ-ODN pre-treatment followed by T/HS with resuscitation with IL6T/HS with resuscitation with IL69528013We previously demonstrated in rodents that T/HS results in liver injury that can be prevented by IL-6 administration at the start of...E-GEOD-27978/profile/8773/arrayexpressuploader115liverJun.26, 2015FalseE-GEOD-27978_A-AFFY-25il-6-mediated-activation-of-stat3-prevents-traumahIL-6-mediated activation of STAT3α prevents trauma/hemorrhagic shock-induced liver inflammationJan.12, 2015We previously demonstrated in rodents that T/HS results in liver injury that can be prevented by IL-6 administration at the start of resuscitation, however the mechanism(s) for the IL-6 protective effect is not fully known. We used microarrays to detail the global gene expression in response to T/HS and the effect of IL-6 on this model with and without pharmacologic blockade of Stat3-mediated IL-6 and identified distinct members of the inflammasome de-regulated during T/HS that normalized with IL-6 administration during resuscitation. Adult male rats were subjected to either sham procedure, a trauma plus hemorrhagic shock (T/HS) procedure, T/HS with IL-6 versus PBS during resuscitation, or pretreatment with pharmacologic blockade of Stat3-mediated IL6 via GQ-ODN and after procedural completion, animals were sacrificed and livers were taken and homegenized for RNA extraction and microarray analysis using Affymetrix Rat 230A GeneChip® arrayhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27978http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27978/samples/