{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 6402, "factors": [{"GSM688700 1": {}}, {"GSM688701 1": {}}, {"GSM688702 1": {}}, {"GSM688703 1": {}}, {"GSM688704 1": {}}, {"GSM688705 1": {}}, {"GSM688706 1": {}}, {"GSM688707 1": {}}, {"GSM688708 1": {}}, {"GSM688709 1": {}}, {"GSM688710 1": {}}, {"GSM688711 1": {}}, {"GSM688712 1": {}}, {"GSM688713 1": {}}, {"GSM688714 1": {}}, {"GSM688715 1": {}}], "pop_total": 0, "platform": 6, "summary_wrapped": "c-Myc is one of key players that are crucially involved in maintaining the undifferentiated state and the self-renewal of ESCs. To...", "pubmed_id": 21726832, "geo_gse_id": "E-GEOD-27881", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 16, "tags": ["protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "effect-of-ablation-of-max-gene-expression-on-es-ce", "geo_id_plat": "E-GEOD-27881_A-AFFY-45", "name": "Effect of ablation of Max gene expression on ES cells cultured under conventional or 2i/Nam condition.", "created": "Nov.11, 2014", "summary": "c-Myc is one of key players that are crucially involved in maintaining the undifferentiated state and the self-renewal of ESCs. To understand the mechanism by which c-Myc helps preserve these prominent characteristics of ESCs, we generated null-ES cells for the Max gene, which encodes the best characterized partner protein for all Myc family proteins. Although Myc/Max complexes have been widely regarded as crucial regulators of the ESC status, our data reveal that ESCs do not absolutely require these complexes in so-called ground state or related conditons and that this requirement is restricted to conventional ES culture conditions without using a MAPK inhibitor. Simply Dox-treated or Nanog (WT or D67G mutant)-rescued Max-null ESCs which were cultured under conventional culture condition and 2i/Nam-treated completely Max-null ES cells from which Dox-regulatable cDNA was removed were used for RNA source. Samples from Dox-untreated Max-null ESCs cultured under conventional culture condition were used as reference samples for Dox-treated cells and Nanog-rescued cells, while a sample from wild-type ESCs cultured under 2i/Nam condition was used as a reference sample for completely Max-null ES cells cultured with 2i/Nam.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27881", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27881/samples/"}