{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6397, "factors": [{"GSM686769": {"GENDER": "female", "GENOTYPE": "Ncstn flox/flox"}}, {"GSM686770": {"GENDER": "male", "GENOTYPE": "Ncstn flox/flox"}}, {"GSM686770": {"GENDER": "male", "GENOTYPE": "Ncstn flox/flox"}}, {"GSM686769": {"GENDER": "female", "GENOTYPE": "Ncstn flox/flox"}}, {"GSM686769": {"GENDER": "female", "GENOTYPE": "Ncstn flox/flox"}}, {"GSM686769": {"GENDER": "female", "GENOTYPE": "Ncstn flox/flox"}}, {"GSM686778": {"GENDER": "female", "GENOTYPE": "Ncstn flox/flox Mx1-cre+"}}, {"GSM686779": {"GENDER": "male", "GENOTYPE": "Ncstn flox/flox Mx1-cre+"}}, {"GSM686779": {"GENDER": "male", "GENOTYPE": "Ncstn flox/flox Mx1-cre+"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Notch signaling is one of the central regulators of differentiation in a variety of organisms and tissue types. Within the hematopoietic...", "pubmed_id": 21562564, "geo_gse_id": "E-GEOD-27811", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 9, "tags": ["anterior", "bone", "bone marrow", "cell", "central", "hematopoietic system", "notch", "pharynx"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-lsk-wt-gmp-wt-and-gmp-ncstnko", "geo_id_plat": "E-GEOD-27811_A-AFFY-45", "name": "Expression data from LSK WT, GMP WT and GMP NcstnKO", "created": "Nov.11, 2014", "summary": "Notch signaling is one of the central regulators of differentiation in a variety of organisms and tissue types. Within the hematopoietic system, Notch is essential for the emergence of definitive HSC during fetal life and controls adult HSC differentiation to the T-cell lineage. Notch activation is controlled by the gamma-secretase complex complex, composed of presenilin, nicastrin (Ncstn), anterior pharynx-1 (Aph1), and presenilin enhancer-2 To determine other role of Notch signaling in HSC we designed a conditional mouse model of Nicastrin deletion. WT and KO HSC were analysed to determined genes controlled by Notch signaling. Bone marrow lineage negative, cKit+, Sca1+ and lineage negative, cKit+, Sca1- CD34+ FcgammaRII/III+ cells were sorted from WT and Ncstn KO littermates for RNA extraction and hybridization on Affymetrix microarrays", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27811", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27811/samples/"}