ArrayExpress Uploader06396maleMx1-cre+femaleMx1-cre+maleMx1-cre+femalelox-stop-lox N1-IC Mx1-cre+femalelox-stop-lox N1-IC Mx1-cre+femalelox-stop-lox N1-IC Mx1-cre+arrayexpress_sid6Notch signaling is one of the central regulators of differentiation in a variety of organisms and tissue types. Within the hematopoietic...21562564E-GEOD-27799/profile/8773/arrayexpressuploader26anteriorbonebone marrowcellcentralhematopoietic systemnotchpharynxDec.12, 2014Falseexpression-data-from-lsk-wt-and-lsk-n1-cE-GEOD-27799_A-AFFY-45Expression data from LSK WT and LSK N1-C+Nov.11, 2014Notch signaling is one of the central regulators of differentiation in a variety of organisms and tissue types. Within the hematopoietic system, Notch is essential for the emergence of definitive HSC during fetal life and controls adult HSC differentiation to the T-cell lineage. Notch activation is controlled by the gamma-secretase complex complex, composed of presenilin, nicastrin (Ncstn), anterior pharynx-1 (Aph1), and presenilin enhancer-2 To determine other role of Notch signaling in HSC we designed a conditional mouse model of Notch activation. WT and N1-IC+ HSC were analysed to determined genes controlled by Notch signaling. Bone marrow lineage negative, cKit+, Sca1+ cells were sorted from WT and N1-IC+ littermates for RNA extraction and hybridization on Affymetrix microarrayshttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27799mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27799/samples/