{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM685456": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6", "TISSUE": "stroma", "GENOTYPE/VARIATION": "KrasTgfbr2wt"}}, {"GSM685456": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6", "TISSUE": "stroma", "GENOTYPE/VARIATION": "KrasTgfbr2wt"}}, {"GSM685456": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6", "TISSUE": "stroma", "GENOTYPE/VARIATION": "KrasTgfbr2wt"}}, {"GSM685459": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6 x Tgfbr2 flox/flox", "TISSUE": "stroma", "GENOTYPE/VARIATION": "KrasTgfbr2-/-"}}, {"GSM685459": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6 x Tgfbr2 flox/flox", "TISSUE": "stroma", "GENOTYPE/VARIATION": "KrasTgfbr2-/-"}}, {"GSM685459": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6 x Tgfbr2 flox/flox", "TISSUE": "stroma", "GENOTYPE/VARIATION": "KrasTgfbr2-/-"}}, {"GSM685459": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6 x Tgfbr2 flox/flox", "TISSUE": "stroma", "GENOTYPE/VARIATION": "KrasTgfbr2-/-"}}, {"GSM685463": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6", "TISSUE": "lung tumor", "GENOTYPE/VARIATION": "KrasTgfbr2wt"}}, {"GSM685463": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6", "TISSUE": "lung tumor", "GENOTYPE/VARIATION": "KrasTgfbr2wt"}}, {"GSM685463": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6", "TISSUE": "lung tumor", "GENOTYPE/VARIATION": "KrasTgfbr2wt"}}, {"GSM685466": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6 x Tgfbr2 flox/flox", "TISSUE": "lung tumor", "GENOTYPE/VARIATION": "KrasTgfbr2-/-"}}, {"GSM685466": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6 x Tgfbr2 flox/flox", "TISSUE": "lung tumor", "GENOTYPE/VARIATION": "KrasTgfbr2-/-"}}, {"GSM685466": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6 x Tgfbr2 flox/flox", "TISSUE": "lung tumor", "GENOTYPE/VARIATION": "KrasTgfbr2-/-"}}, {"GSM685466": {"STRAIN BACKGROUND": "LSL-KrasG12D x C57/Bl6 x Tgfbr2 flox/flox", "TISSUE": "lung tumor", "GENOTYPE/VARIATION": "KrasTgfbr2-/-"}}], "id": 6385, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Recent data suggests that repression of the Type II TGF-B Receptor (Tgfr2) repression in human lung adenocarcinoma is important for...", "geo_gse_id": "E-GEOD-27675", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 14, "tags": ["adenocarcinoma", "cancer", "cell", "compartment", "lung", "lung adenocarcinoma", "lung cancer", "stromal cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-lung-tumor-and-stromal-cells", "geo_id_plat": "E-GEOD-27675_A-AFFY-45", "name": "Expression data from lung tumor and stromal cells of KrasTgfbr2 -/- mouse model", "created": "Nov.11, 2014", "summary": "Recent data suggests that repression of the Type II TGF-B Receptor (Tgfr2) repression in human lung adenocarcinoma is important for progression from noninvasive to invasive adenocarcinoma. To test this hypothesis in a animal model of non-invasive lung cancer, we generated an inducible, lung specific Tgfbr2 knockout model in the oncogenic Kras mouse. LSL-KrasG12D positive mice were simultaneously backcrossed to C57/Bl6 mice and to the Tgfbr2 flox/flox mice. To induce tumors, 100 ul of saline containing 3x10e10 particles of an adenovirus containing the Cre recombinase (Ad.Cre) was administered to each LSL-KrasG12D mouse intra-nasally. We evaluated the tumor microenvironment response to Tgfbr2 deficient tumor cells.  We compared lung tumor cell and stromal cell transcriptional profiles from five-week KrasTgfbr2 -/-  and nine-week KrasTgfbr2 WT mice. We used mice at these time points to allow comparison of the stromal compartment of similarly advanced tumors.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27675", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27675/samples/"}