{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM684887": {"TRANSFECTION": "control non-targetting siCO"}}, {"GSM684888": {"TRANSFECTION": "miR127"}}], "id": 6382, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27642", "summary_wrapped": "The molecular mechanisms of acute lung injury are incompletely understood. MicroRNAs (miRNAs) are crucial biological regulators that act...", "pubmed_id": 22287715, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 2, "tags": ["cytokine", "genome", "lung", "macrophage", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "mirna-127-inhibits-igg-immune-complex-induced-lung", "geo_id_plat": "E-GEOD-27642_A-AFFY-45", "name": "MiRNA-127 Inhibits IgG Immune Complex-induced Lung Inflammation by Targeting IgG Fc\u03b3 Receptor I", "created": "Nov.11, 2014", "summary": "The molecular mechanisms of acute lung injury are incompletely understood. MicroRNAs (miRNAs) are crucial biological regulators that act by suppressing their target genes and are involved in a variety of pathophysiologic processes. MiR-127 appeared to be down-regulated during lung injury. We set out to investigate the role of miR-127 in lung injury and inflammation. Expression of miR-127 significantly reduced cytokine release by macrophages. Looking into the mechanisms of the regulation of inflammation by miR-127, we found that IgG Fc\u03b3 Receptor I (Fc\u03b3RI/CD64) was a target of miR-127, as evidenced by reduced CD64 protein expression in macrophages over-expressing miR-127. Furthermore, miR-127 significantly reduced the luciferase activity with a reporter construct containing the native 3\u2019-UTR of CD64. Importantly, we demonstrated that miR-127 attenuated lung inflammation in an IgG immune complex (IgG IC) model in vivo. Collectively, these data show that miR-127 targets macrophage CD64 expression and promotes the reduction of lung inflammation. Understanding how miRNAs regulate lung inflammation may represent an attractive way to control inflammation induced by infectious or non-infectious lung injury. MH.S-miR127 and MH.S-Sico cells were cultured for RNA extraction.Total RNA were assessed for quality with Agilent 2100 Bioanalyzer G2939A (Agilent Technologies,Santa Clara, CA)) and Nanodrop 8000 spectrophotometer (Thermo Scientific/Nanodrop, Wilmington, DE). Hybridization targets were prepared with MessageAmp\u2122 Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA, hybridized to GeneChip\u00ae Mouse Genome 430 2.0 arrays in Affymetrix  GeneChip\u00ae hybridization oven 645, washed in Affymetrix GeneChip\u00ae  Fluidics Station 450 and scanned with Affymetrix GeneChip\u00ae Scanner 7G according to standard Affymetrix GeneChip\u00ae Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA).", "geo_gse_id": "E-GEOD-27642", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27642/samples/"}