ArrayExpress Uploaderarrayexpress_sidmouseLgr5 HighApril_2010Lgr5 LowApril_2010EphB2 HighFeb_2009EphB2 MediumFeb_2009EphB2 LowFeb_2009EphB2 HighMarch_2009EphB2 MediumMarch_2009EphB2 LowMarch_2009637906Using EphB2 or the ISC marker Lgr5, we have FACS-purified and profiled intestinal stem cells (ISCs), crypt proliferative progenitors and...E-GEOD-27605/profile/8773/arrayexpressuploader28cancercellcolorectal cancerdiseasestem cellDec.12, 2014FalseE-GEOD-27605_A-AFFY-45the-intestinal-stem-cell-signature-identifies-coloThe intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapseNov.11, 2014Using EphB2 or the ISC marker Lgr5, we have FACS-purified and profiled intestinal stem cells (ISCs), crypt proliferative progenitors and late transient amplifying cells to define a gene expression program specific for normal ISCs. A frequent complication in colorectal cancer (CRC) is regeneration of the tumor after therapy. The intestinal stem cell signature predicts disease relapse in CRC and identifies a stem cell-like population that displays robust tumor- initiating capacity in immunodeficient mice as well as long-term self-renewal potential. We FACS purified mouse intestinal crypt cells according to their EphB2 or Lgr5 contents. We used Affymetrix chips to hybridize 2 samples from EphB2 high, 2 samples from EphB2 medium and 2 samples from EphB2 low cells (one sample from each group in a first hybridization on February 2009 plus an additional sample from each group on March 2009). Additionally, we hybridized one sample from Lgr5-EGFP high and one sample from Lgr5-EGFP low cells, obtained from Lgr5-EGFP knock-in mice (Barker et al., 2007).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27605http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27605/samples/