{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM684317": {"GROUP": "Lgr5 High", "HYBRIDIZATION_DATE": "April_2010"}}, {"GSM684318": {"GROUP": "Lgr5 Low", "HYBRIDIZATION_DATE": "April_2010"}}, {"GSM684319": {"GROUP": "EphB2 High", "HYBRIDIZATION_DATE": "Feb_2009"}}, {"GSM684320": {"GROUP": "EphB2 Medium", "HYBRIDIZATION_DATE": "Feb_2009"}}, {"GSM68432": {"GROUP": "EphB2 Low", "HYBRIDIZATION_DATE": "Feb_2009"}}, {"GSM684322": {"GROUP": "EphB2 High", "HYBRIDIZATION_DATE": "March_2009"}}, {"GSM684323": {"GROUP": "EphB2 Medium", "HYBRIDIZATION_DATE": "March_2009"}}, {"GSM684324": {"GROUP": "EphB2 Low", "HYBRIDIZATION_DATE": "March_2009"}}], "id": 6379, "pop_total": 0, "platform": 6, "summary_wrapped": "Using EphB2 or the ISC marker Lgr5, we have FACS-purified and profiled intestinal stem cells (ISCs), crypt proliferative progenitors and...", "geo_gse_id": "E-GEOD-27605", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 8, "tags": ["cancer", "cell", "colorectal cancer", "disease", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-27605_A-AFFY-45", "slug": "the-intestinal-stem-cell-signature-identifies-colo", "geo_gds_id": "", "name": "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse", "created": "Nov.11, 2014", "summary": "Using EphB2 or the ISC marker Lgr5, we have FACS-purified and profiled intestinal stem cells (ISCs), crypt proliferative progenitors and late transient amplifying cells to define a gene expression program specific for normal ISCs. A frequent complication in colorectal cancer (CRC) is regeneration of the tumor after therapy. The intestinal stem cell signature predicts disease relapse in CRC and identifies a stem cell-like population that displays robust tumor- initiating capacity in immunodeficient mice as well as long-term self-renewal potential. We FACS purified mouse intestinal crypt cells according to their EphB2 or Lgr5 contents. We used Affymetrix chips to hybridize 2 samples from EphB2 high, 2 samples from EphB2 medium and 2 samples from EphB2 low cells (one sample from each group in a first hybridization on February 2009 plus an additional sample from each group on March 2009). Additionally, we hybridized one sample from Lgr5-EGFP high and one sample from Lgr5-EGFP low cells, obtained from Lgr5-EGFP knock-in mice (Barker et al., 2007).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27605", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27605/samples/"}