ArrayExpress Uploaderarrayexpress_sid6375HMGN1 knock outprimary MEFsHMGN1 knock outprimary MEFsHMGN1 knock outprimary MEFsHMGN1 WTprimary MEFsHMGN1 WTprimary MEFsHMGN1 WTprimary MEFsHMGN3 knock outprimary MEFsHMGN3 knock outprimary MEFsHMGN3 knock outprimary MEFsHMGN3 WTprimary MEFsHMGN3 WTprimary MEFsHMGN3 WTprimary MEFsHMGN5 knock outprimary MEFsHMGN5 knock outprimary MEFsHMGN5 knock outprimary MEFsHMGN5 WTprimary MEFsHMGN5 WTprimary MEFsHMGN5 WTprimary MEFsempty vector OESV40 transformed MEFsempty vector OESV40 transformed MEFsempty vector OESV40 transformed MEFsHMGN1 OESV40 transformed MEFsHMGN1 OESV40 transformed MEFsHMGN1 OESV40 transformed MEFsHMGN2 OESV40 transformed MEFsHMGN2 OESV40 transformed MEFsHMGN2 OESV40 transformed MEFsHMGN3a OESV40 transformed MEFsHMGN3a OESV40 transformed MEFsHMGN3a OESV40 transformed MEFsHMGN5 OESV40 transformed MEFsHMGN5 OESV40 transformed MEFsHMGN5 OESV40 transformed MEFsHMGN5SE OESV40 transformed MEFsHMGN5SE OESV40 transformed MEFsHMGN5SE OESV40 transformed MEFsHMGN1_N2swapSV40 transformed MEFsHMGN1_N2swapSV40 transformed MEFsHMGN1_N2swapSV40 transformed MEFsHMGN1_N3swapSV40 transformed MEFsHMGN1_N3swapSV40 transformed MEFsHMGN1_N3swapSV40 transformed MEFsempty vector OEMIN6 cellsempty vector OEMIN6 cellsempty vector OEMIN6 cellsHMGN1 OEMIN6 cellsHMGN1 OEMIN6 cellsHMGN1 OEMIN6 cellsHMGN3a OEMIN6 cellsHMGN3a OEMIN6 cellsHMGN3a OEMIN6 cells06HMGN (high mobility group N) is a family of intrinsically disordered nuclear proteins that binds to nucleosomes, alters the structure of...21278158E-GEOD-27546/profile/8773/arrayexpressuploader251chromatingenomeproteinDec.12, 2014Falseeffects-of-hmgn-variants-on-cellular-transcriptionE-GEOD-27546_A-AFFY-45Effects of HMGN variants on cellular transcription profileNov.11, 2014HMGN (high mobility group N) is a family of intrinsically disordered nuclear proteins that binds to nucleosomes, alters the structure of chromatin and affects transcription. A major unresolved question is the extent of functional specificity, or redundancy, between the various members of the HMGN protein family. Here we analyze the transcriptional profile of cells in which the expression of various HMGN proteins has been either deleted or doubled. The results reveal an HMGN-variant specific effect on the fidelity of the cellular transcription profile, indicating that functionally, the various HMGN subtypes are not fully redundant. RNA was collected from either primary knock-out MEFs or SV40-transformed MEFs and MIN6 cells over expressing various HMGN proteins and mutants and hybridized to Affymetrix arrays. We obtained a double ammount of HMGN proteins in MEFs and MIN6 cells by retroviral infection and subsequent selection procedure. We collected all infected cells (pools, not clones) in order to eliminate the effect of viral integration in the genome.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27546mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27546/samples/