{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM679719": {"CELL LINE": "B6TC", "GENOTYPE": "GFP+ and estrogen-independent"}}, {"GSM679719": {"CELL LINE": "B6TC", "GENOTYPE": "GFP+ and estrogen-independent"}}, {"GSM679719": {"CELL LINE": "B6TC", "GENOTYPE": "GFP+ and estrogen-independent"}}, {"GSM679722": {"CELL LINE": "MDA231", "GENOTYPE": "ER negative MDA-MB-231/GFP/Neo cells resistant to G418"}}, {"GSM679722": {"CELL LINE": "MDA231", "GENOTYPE": "ER negative MDA-MB-231/GFP/Neo cells resistant to G418"}}, {"GSM679722": {"CELL LINE": "MDA231", "GENOTYPE": "ER negative MDA-MB-231/GFP/Neo cells resistant to G418"}}, {"GSM679725": {"CELL LINE": "ZR75", "GENOTYPE": "ER positive ZR-75-1/GFP/puro"}}, {"GSM679725": {"CELL LINE": "ZR75", "GENOTYPE": "ER positive ZR-75-1/GFP/puro"}}, {"GSM679725": {"CELL LINE": "ZR75", "GENOTYPE": "ER positive ZR-75-1/GFP/puro"}}], "id": 3979, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "The origin and the contribution of breast tumor heterogeneity to its progression are not clear. We investigated the effect of a growing...", "geo_gse_id": "E-GEOD-27515", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 9, "tags": ["bone", "bone marrow", "breast", "breast tumor", "cancer", "cell", "estrogen", "line"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "isolation-and-characterization-of-a-metastatic-hyb", "geo_id_plat": "E-GEOD-27515_A-AFFY-44", "name": "Isolation and characterization of a metastatic hybrid cell line generated by ER negative and ER positive breast cancer cells in mouse bone marrow", "created": "Sep.15, 2014", "summary": "The origin and the contribution of breast tumor heterogeneity to its progression are not clear. We investigated the effect of a growing orthotopic tumor formed by an aggressive estrogen receptor (ER)-negative breast cancer cell line on the metastatic potential of a less aggressive ER-positive breast cancer cell line for the elucidation of how the presence of heterogeneous cancer cells might affect each other\u2019s metastatic behavior. ER positive ZR-75-1/GFP/puro cells, resistant to puromycin and non-tumorigenic/non-metastatic without exogenous estrogen supplementation, were injected intracardiacally into mice bearing growing orthotopic tumors, formed by ER negative MDA-MB-231/GFP/Neo cells resistant to G418. A variant cell line B6, containing both estrogen-dependent and -independent cells, were isolated from GFP expressing cells in the bone marrow and re-inoculated in nude mice to generate an estrogen-independent cell line B6TC.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27515", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27515/samples/"}