{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677189": {"DISEASE STATE": "remitted schizophrenia patient"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677182": {"DISEASE STATE": "healthy control"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}, {"GSM677173": {"DISEASE STATE": "acutely admitted, severely psychotic schizophrenia patient"}}], "id": 2377, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Recent studies have suggested that deregulated AKT1 signaling is associated with schizophrenia. We hypothesized that if this is indeed...", "geo_gse_id": "E-GEOD-27383", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 72, "tags": ["genome", "peripheral", "schizophrenia"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-27383_A-AFFY-44", "slug": "marked-reduction-of-akt1-expression-and-deregulati", "geo_gds_id": "", "name": "Marked Reduction of AKT1 Expression and Deregulation of AKT1-associated Pathways in Peripheral Blood Mononuclear Cells of Schizophrenia Patients", "created": "Jul.12, 2014", "summary": "Recent studies have suggested that deregulated AKT1 signaling is associated with schizophrenia. We hypothesized that if this is indeed the case, we should observe both decreased AKT1 expression as well as deregulation of AKT1 regulated pathways in Peripheral Blood Mononuclear Cells (PBMCs) of schizophrenia patients. We therefore examined PBMC expression levels of AKT1 in schizophrenia  patients  versus controls, and examined whether functional biological processes in which AKT1 plays an important role are deregulated in schizophrenia patients.   We performed a case-control study, investigating whole-genome PBMC gene expression in male, recent onset (<5 years) schizophrenia patients (N=43) as compared to controls (N=29). Genes, differentially expressed between patients and controls were identified using ANOVA with Benjamini-Hochberg correction (false discovery rate (FDR)= 0.05).   Functional aspects of the deregulated set of genes were investigated with the Ingenuity Pathway Analysis (IPA) Software Tool. From each participant 30 ml of blood was drawn into heparinized tubes. All blood samples were obtained between 10.00 and 11.00 am to minimize diurnal variation. PBMC\u2019s were isolated by Ficoll gradient separation, started within 20 minutes after the drawing of blood and performed with minimum time variation. Cells were subsequently disrupted (Qiashredder kit; Qiagen), and RNA was isolated (RNeasy minikit; Qiagen) with an additional DNAse digestion step (RNase-free DNase set; Qiagen), all according to the manufacturer\u2019s protocol, diluted in nuclease free water, and frozen at \u201380 C before use. Before freezing, RNA purity and quantity was assessed with the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies). After thawing the isolated RNA was biotinylated into cRNA using the One-Cycle Target Labeling and Control Reagents Kit (Affymetric Co) according to the manufacturer\u2019s protocol. Before hybridisation RNA quality and integrity was assessed using the Agilent 2100 BioAnalyzer (Agilent). Biotinylated cRNA was hybridized to the Affymetrix Human Genome U133 plus 2.0 GeneChip\u00a9 microarray containing 54,675 probe sets (Affymetrix Co). Each sample was individually biotinylated and hybridized to an individual microarray. Biotinylation was performed in batches with randomisation of patients and controls according to their ratios across the batches. The arrays were scanned and analyzed using Affymetrix Microarray Suite 4.2 software. Scanning was performed in three batches with randomization of patients and controls according to their ratios across the batches. A case-control study, investigating whole-genome PBMC gene expression in male, recent onset (<5 years) schizophrenia patients (N=43) as compared to controls (N=29). Patients consisted of two subgroups: acutely admitted, severely psychotic (N=22), and remitted patients (N=21). Genes, differentially expressed between patients and controls were identified using ANOVA with Benjamini-Hochberg correction (false discovery rate (FDR)= 0.05).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27383", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27383/samples/"}