ArrayExpress Uploader0mouselung, lymphocytesbronchial lymphnodes, lymphocytesbronchial lymphnodes, lymphocyteslung, lymphocytesbronchial lymphnodes, lymphocyteslung, lymphocytes6366arrayexpress_sid6Role for naturally occurring CD4+CD25+Foxp3+ regulatory T cells (nTregs) in counterbalancing this process. Using a transgenic murine...E-GEOD-27379/profile/8773/arrayexpressuploader16celldiseaseinfluenzalunglung diseaselymphspleenDec.12, 2014Falsecd8-t-cell-mediated-lung-inflammationE-GEOD-27379_A-AFFY-45CD8+ T cell mediated lung inflammationNov.11, 2014Role for naturally occurring CD4+CD25+Foxp3+ regulatory T cells (nTregs) in counterbalancing this process. Using a transgenic murine model for autoimmune-mediated lung disease, we demonstrated that, despite pulmonary inflammation, lung-specific CD8+ T cells can reside quiescently in close proximity to self-antigen. Whereas self-reactive CD8+ T cells in the inflamed lung and lung-draining lymph nodes down-regulated the expression of effector molecules, those located in the spleen appeared to be partly antigen-experienced and displayed a memory-like phenotype. Since ex vivo-reisolated self-reactive CD8+ T cells were very well capable to respond to the antigen in vitro, we investigated a possible contribution of nTregs to the immune control over autoaggressive CD8+ T cells in the lung. We isolated antigen-specifc CD8+ T-cells from lungs and bronchial lymphnodes derived from chronic diseased mice (SPC-HAxCL4), healthy control mice (CL4) and acute influenza infected control mice (CL4+IAV) and perormed mRNA expression profiling of isolated CD8+ T cells. Each group represents a pool of at least n=4 animals. CD8+ T cell type comparison; lung disease state analysishttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27379http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27379/samples/