{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3964, "factors": [{"GSM674835": {"SAMPLE TYPE": "peripheral blood lymphocytes isolated prior to activation", "INDIVIDUAL": "donor 1", "TREATMENT": "control", "TIME": "0"}}, {"GSM674836": {"SAMPLE TYPE": "peripheral blood lymphocytes isolated and activated in vitro for 6 hrs", "INDIVIDUAL": "donor 1", "TREATMENT": "BrHPP/IL2", "TIME": "6 h"}}, {"GSM674837": {"SAMPLE TYPE": "peripheral blood lymphocytes isolated and activated in vitro for 7 days", "INDIVIDUAL": "donor 1", "TREATMENT": "BrHPP/IL2", "TIME": "7 d"}}, {"GSM674838": {"SAMPLE TYPE": "peripheral blood lymphocytes isolated prior to activation", "INDIVIDUAL": "donor 2", "TREATMENT": "control", "TIME": "0"}}, {"GSM674839": {"SAMPLE TYPE": "peripheral blood lymphocytes isolated and activated in vitro for 6 hrs", "INDIVIDUAL": "donor 2", "TREATMENT": "BrHPP/IL2", "TIME": "6 h"}}, {"GSM674840": {"SAMPLE TYPE": "peripheral blood lymphocytes isolated and activated in vitro for 7 days", "INDIVIDUAL": "donor 2", "TREATMENT": "BrHPP/IL2", "TIME": "7 d"}}, {"GSM67484": {"SAMPLE TYPE": "peripheral blood lymphocytes isolated prior to activation", "INDIVIDUAL": "donor 3", "TREATMENT": "control", "TIME": "0"}}, {"GSM674842": {"SAMPLE TYPE": "peripheral blood lymphocytes isolated and activated in vitro for 6 hrs", "INDIVIDUAL": "donor 3", "TREATMENT": "BrHPP/IL2", "TIME": "6 h"}}, {"GSM674843": {"SAMPLE TYPE": "peripheral blood lymphocytes isolated and activated in vitro for 7 days", "INDIVIDUAL": "donor 3", "TREATMENT": "BrHPP/IL2", "TIME": "7 d"}}, {"GSM674844": {"SAMPLE TYPE": "peripheral blood lymphocytes isolated prior to activation", "INDIVIDUAL": "donor 4", "TREATMENT": "control", "TIME": "0"}}, {"GSM674845": {"SAMPLE TYPE": "peripheral blood lymphocytes isolated and activated in vitro for 6 hrs", "INDIVIDUAL": "donor 4", "TREATMENT": "BrHPP/IL2", "TIME": "6 h"}}, {"GSM674846": {"SAMPLE TYPE": "peripheral blood lymphocytes isolated and activated in vitro for 7 days", "INDIVIDUAL": "donor 4", "TREATMENT": "BrHPP/IL2", "TIME": "7 d"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "We used microarrays to detail the global programme of gene expression by circulating TCRVgamma9+ gamma delta T cells isolated from...", "pubmed_id": 21968650, "geo_gse_id": "E-GEOD-27291", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 4, "sample_count": 12, "tags": ["cell", "chemokine", "cytokine", "peripheral"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-human-tcrvg9-positive-gamma-d", "geo_id_plat": "E-GEOD-27291_A-AFFY-44", "name": "Expression data from human TCRVg9-positive gamma delta T lymphocytes", "created": "Sep.15, 2014", "summary": "We used microarrays to detail the global programme of gene expression by circulating TCRVgamma9+ gamma delta T cells isolated from healthy individuals,tested either as resting cells or cells activated by phosphoantigen BrHPP and IL-2at an early(+6hrs)  and a late (+7days) timepoint. We find that with more \u201cNK cell\u201d genes than alphabeta T cells and more \u201cT cell\u201d genes than NK cells, the circulating TCRVgamma9+ gamma delta T cells cells have a hybrid transcriptome. The gene signature of the activated cells recapitulates their physiological functions: Th1 cytokine, chemokine and cytotoxic activities at first and mitotic activity at later time points. The gene expression pattern of activated normal gamma delta T cells is nevertheless clearly distinctive from that of NK/T and peripheral T cell lymphomas of the gamma delta subtype. Human TCRVg9positive gamma delta T cells were isolated  from PBMC by cell sorting (>98% purity) and activated for RNA extraction and hybridization on Affymetrix microarrays. Samples comprise cells before activation (control time 0), early after activation with BrHPP/IL2 (+6 hours) and at a later timepoint of the activated in vitro culture  with BrHPP/IL2 (day 7).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27291", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27291/samples/"}