{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM667580": {"CONDITION": "control siRNA"}}, {"GSM667580": {"CONDITION": "control siRNA"}}, {"GSM667580": {"CONDITION": "control siRNA"}}, {"GSM667583": {"CONDITION": "Dcp1a siRNA"}}, {"GSM667583": {"CONDITION": "Dcp1a siRNA"}}, {"GSM667583": {"CONDITION": "Dcp1a siRNA"}}, {"GSM667586": {"CONDITION": "Dcp2 siRNA"}}, {"GSM667586": {"CONDITION": "Dcp2 siRNA"}}, {"GSM667586": {"CONDITION": "Dcp2 siRNA"}}, {"GSM667589": {"CONDITION": "Dcp1a+Dcp2 siRNA"}}, {"GSM667589": {"CONDITION": "Dcp1a+Dcp2 siRNA"}}, {"GSM667589": {"CONDITION": "Dcp1a+Dcp2 siRNA"}}], "id": 6349, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27049", "summary_wrapped": "Oocyte maturation is accompanied by a transition from mRNA stability to instability.  We investigated the role of DCP1A and DCP2,...", "pubmed_id": 23136299, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 12, "tags": ["cell", "genome", "oocyte"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "effects-of-dcp1a-and-dcp2-knockdown-during-mouse-o", "geo_id_plat": "E-GEOD-27049_A-AFFY-45", "name": "Effects of Dcp1a and Dcp2 knockdown during mouse oocyte maturation", "created": "Nov.11, 2014", "summary": "Oocyte maturation is accompanied by a transition from mRNA stability to instability.  We investigated the role of DCP1A and DCP2, proteins responsible for mRNA decapping,  in mRNA destabilization during mouse oocyte maturation. siRNA-mediated knockdown of both Dcp1a and Dcp2 transcripts prior to initiation of maturation inhibited the maturation-associated increase of DCP1A and DCP2, stabilized a set of maternal mRNAs that are normally degraded during maturation, and inhibited development beyond the 2-cell stage, likely a consequence of failure to activate fully the zygotic genome. Total RNA from 30 MII eggs was used in each sample. Three independent biological replicates were analyzed for each condition.", "geo_gse_id": "E-GEOD-27049", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27049/samples/"}