6ArrayExpress Uploader0mousecontrol siRNAcontrol siRNAcontrol siRNADcp1a siRNADcp1a siRNADcp1a siRNADcp2 siRNADcp2 siRNADcp2 siRNADcp1a+Dcp2 siRNADcp1a+Dcp2 siRNADcp1a+Dcp2 siRNA6349arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27049Oocyte maturation is accompanied by a transition from mRNA stability to instability. We investigated the role of DCP1A and DCP2,...23136299/profile/8773/arrayexpressuploader112cellgenomeoocyteDec.12, 2014Falseeffects-of-dcp1a-and-dcp2-knockdown-during-mouse-oE-GEOD-27049_A-AFFY-45Effects of Dcp1a and Dcp2 knockdown during mouse oocyte maturationNov.11, 2014Oocyte maturation is accompanied by a transition from mRNA stability to instability. We investigated the role of DCP1A and DCP2, proteins responsible for mRNA decapping, in mRNA destabilization during mouse oocyte maturation. siRNA-mediated knockdown of both Dcp1a and Dcp2 transcripts prior to initiation of maturation inhibited the maturation-associated increase of DCP1A and DCP2, stabilized a set of maternal mRNAs that are normally degraded during maturation, and inhibited development beyond the 2-cell stage, likely a consequence of failure to activate fully the zygotic genome. Total RNA from 30 MII eggs was used in each sample. Three independent biological replicates were analyzed for each condition.E-GEOD-27049http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27049/samples/