{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM665382": {"CELL TYPE": "Melan-a NRAS61K cells", "TIME": "6 days"}}, {"GSM665383": {"CELL TYPE": "Melan-a NRAS61K cells", "TIME": "14 days"}}, {"GSM665384": {"CELL TYPE": "Melan-a NRAS61K cells", "TIME": "28 days"}}, {"GSM665385": {"CELL TYPE": "Melan-a NRAS61K-AR cells", "TIME": "10 days"}}], "id": 6346, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Activation of oncogene signaling in primary cells generally results in cellular senescence. This process is not only considered to be...", "geo_gse_id": "E-GEOD-27010", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 4, "tags": ["melanocyte", "melanoma", "metastatic melanoma", "nevus"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "generation-of-highly-tumorigenic-cells-from-n-ras6", "geo_id_plat": "E-GEOD-27010_A-AFFY-45", "name": "Generation of highly tumorigenic cells from N-RAS61K-induced senescent cells", "created": "Nov.11, 2014", "summary": "Activation of oncogene signaling in primary cells generally results in cellular senescence. This process is not only considered to be  tumor-protective, but also irreversible. However, if senescence induction can be circumvented, e.g. by additional genetic or epigenetic changes, tumor progression occurs. An in-vivo example for oncogene-induced senescence are human nevus cells, most of which bear oncogenic mutations in RAS/RAF/MAPK pathway genes. Here, we show that expression of the human melanoma oncogene N-RAS61K in cultured pigment cells initially induces OIS characterized by a highly multinucleated phenotype. Surprisingly, after prolonged periods of oncogene expression, mononucleated cells emerge from the multinucleated cells. They are highly proliferative, anoikis-resistent and induce fast growing and metastatic melanoma upon transplantation into nude mice. During long-term oncogene expression and the corresponding development of anoikis iresistance, expression of melanocyte-specific genes is lost. Our data demonstrate that the induction of oncogene-induced senescence is not just a failsafe escape mechanism, but also provides a source for highly aggressive tumor cells. total samples analysed are 4", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27010", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-27010/samples/"}