ArrayExpress Uploader03936H9 human embryonic stem cellsH9 human embryonic stem cellsH9 human embryonic stem cellshSOX17-2 derived stage 1 endodermhSOX17-2 derived stage 1 endodermhSOX17-2 derived stage 1 endodermhSOX17-2 derived stage 2 endodermhSOX17-2 derived stage 2 endodermhSOX17-2 derived stage 2 endodermarrayexpress_sid4Using homologous recombination in human ESC, we inserted an enhanced green fluorescent protein (eGFP) transgene into a locus encoding a...21362573E-GEOD-26862/profile/8773/arrayexpressuploader19cellendodermproteinDec.12, 2014Falseexpression-data-of-human-egfp-sox17-endoderm-cellsE-GEOD-26862_A-AFFY-44Expression data of human eGFP-SOX17+ endoderm cellsSep.15, 2014Using homologous recombination in human ESC, we inserted an enhanced green fluorescent protein (eGFP) transgene into a locus encoding a postulated marker of human endoderm, SOX17 in H9 human embryonic stem cells. This allowed purification of SOX17+ hESC endodermal progeny by fluorescence activated cell sorting (FACS) to generate microarray gene expression profile. Using Wnt3 and Activin to differentiate hSOX17-2 to stage 1 cells, and subsequently FGF10 and cyclopamine to stage 2 cells, we isolated eGFP+ cells by FACS at each stage, performed microarray analysis.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26862humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26862/samples/