{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3936, "factors": [{"GSM661349": {"CELL TYPE": "H9 human embryonic stem cells"}}, {"GSM661349": {"CELL TYPE": "H9 human embryonic stem cells"}}, {"GSM661349": {"CELL TYPE": "H9 human embryonic stem cells"}}, {"GSM661352": {"CELL TYPE": "hSOX17-2 derived stage 1 endoderm"}}, {"GSM661352": {"CELL TYPE": "hSOX17-2 derived stage 1 endoderm"}}, {"GSM661352": {"CELL TYPE": "hSOX17-2 derived stage 1 endoderm"}}, {"GSM661355": {"CELL TYPE": "hSOX17-2 derived stage 2 endoderm"}}, {"GSM661355": {"CELL TYPE": "hSOX17-2 derived stage 2 endoderm"}}, {"GSM661355": {"CELL TYPE": "hSOX17-2 derived stage 2 endoderm"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Using homologous recombination in human ESC, we inserted an enhanced green fluorescent protein (eGFP) transgene into a locus encoding a...", "pubmed_id": 21362573, "geo_gse_id": "E-GEOD-26862", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": ["cell", "endoderm", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-of-human-egfp-sox17-endoderm-cells", "geo_id_plat": "E-GEOD-26862_A-AFFY-44", "name": "Expression data of human eGFP-SOX17+ endoderm cells", "created": "Sep.15, 2014", "summary": "Using homologous recombination in human ESC, we inserted an enhanced green fluorescent protein (eGFP) transgene into a locus encoding a postulated marker of human endoderm, SOX17 in H9 human embryonic stem cells. This allowed purification of SOX17+ hESC endodermal progeny by fluorescence activated cell sorting (FACS) to generate microarray gene expression profile. Using Wnt3 and Activin to differentiate hSOX17-2 to stage 1 cells, and subsequently FGF10 and cyclopamine to stage 2 cells,  we isolated eGFP+ cells by FACS at each stage, performed microarray analysis.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26862", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26862/samples/"}