ArrayExpress Uploader0mouseCD4 T-cellsleuckocyte costimulatory blocking antibodies anti-LFA-1, CTLA4Ig, anti-CD40-ligandCD4 T-cellsleuckocyte costimulatory blocking antibodies anti-LFA-1, CTLA4Ig, anti-CD40-ligandCD4 T-cellscontrolCD4 T-cellscontrolCD8 T-cellsleuckocyte costimulatory blocking antibodies anti-LFA-1, CTLA4Ig, anti-CD40-ligandCD8 T-cellsleuckocyte costimulatory blocking antibodies anti-LFA-1, CTLA4Ig, anti-CD40-ligandCD8 T-cellscontrolCD8 T-cellscontrol6331arrayexpress_sid6To elucidate the gene expression “footprint” of antigenically challenged T-cells which had been treated with anti-LFA-1, CTLA4Ig, anti-...E-GEOD-26669/profile/8773/arrayexpressuploader28lymphocyteDec.12, 2014Falseexpression-data-of-alloantigen-stimulated-splenocyE-GEOD-26669_A-AFFY-45Expression data of alloantigen stimulated splenocytes treated with leukocyte costimulatory blockade antibodies or no treatmentNov.11, 2014To elucidate the gene expression “footprint” of antigenically challenged T-cells which had been treated with anti-LFA-1, CTLA4Ig, anti-CD40-ligand antibodies, we performed microarray gene expression analysis comparing the expression profile of costimulatory blockade treated and untreated responder T-cells. Allogeneic murine splenocytes were co-cultured in an vitro set-up for a mixed lymphocyte reaction. For the experimental/treated group, the leuckocyte costimulatory blocking antibodies anti-LFA-1, CTLA4Ig, anti-CD40-ligand were added to the media, the control group did not have any antiodies added to the media. After 5 days of incubation the responder CD4 and CD8 T cells were isolated by FACS and the gene expression was analyzed using affymetrix microarray platforms.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26669http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26669/samples/