{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM656409": {"CELL TYPE": "CD4 T-cells", "AGENT": "leuckocyte costimulatory blocking antibodies anti-LFA-1, CTLA4Ig, anti-CD40-ligand"}}, {"GSM656409": {"CELL TYPE": "CD4 T-cells", "AGENT": "leuckocyte costimulatory blocking antibodies anti-LFA-1, CTLA4Ig, anti-CD40-ligand"}}, {"GSM6564": {"CELL TYPE": "CD4 T-cells", "AGENT": "control"}}, {"GSM6564": {"CELL TYPE": "CD4 T-cells", "AGENT": "control"}}, {"GSM656413": {"CELL TYPE": "CD8 T-cells", "AGENT": "leuckocyte costimulatory blocking antibodies anti-LFA-1, CTLA4Ig, anti-CD40-ligand"}}, {"GSM656413": {"CELL TYPE": "CD8 T-cells", "AGENT": "leuckocyte costimulatory blocking antibodies anti-LFA-1, CTLA4Ig, anti-CD40-ligand"}}, {"GSM656415": {"CELL TYPE": "CD8 T-cells", "AGENT": "control"}}, {"GSM656415": {"CELL TYPE": "CD8 T-cells", "AGENT": "control"}}], "id": 6331, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "To elucidate the gene expression \u201cfootprint\u201d of antigenically challenged T-cells which had been treated with anti-LFA-1, CTLA4Ig, anti-...", "geo_gse_id": "E-GEOD-26669", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 8, "tags": ["lymphocyte"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-of-alloantigen-stimulated-splenocy", "geo_id_plat": "E-GEOD-26669_A-AFFY-45", "name": "Expression data of alloantigen stimulated splenocytes treated with leukocyte costimulatory blockade antibodies or no treatment", "created": "Nov.11, 2014", "summary": "To elucidate the gene expression \u201cfootprint\u201d of antigenically challenged T-cells which had been treated with anti-LFA-1, CTLA4Ig, anti-CD40-ligand antibodies, we performed microarray gene expression analysis comparing the expression profile of costimulatory blockade treated and untreated responder T-cells. Allogeneic murine splenocytes were co-cultured in an vitro set-up for a mixed lymphocyte reaction. For the experimental/treated group, the leuckocyte costimulatory blocking antibodies anti-LFA-1, CTLA4Ig, anti-CD40-ligand were added to the media, the control group did not have any antiodies added to the media. After 5 days of incubation the responder CD4 and CD8 T cells were isolated by FACS and the gene expression was analyzed using affymetrix microarray platforms.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26669", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26669/samples/"}