ArrayExpress Uploaderarrayexpress_sidhuman2Dempty vector control (pIRES2)2DFLAG tagged ARHGAP8 in pIRES23Dempty vector control (pIRES2)3DFLAG tagged ARHGAP8 in pIRES2392304ARHGAP8 was expressed at negligible levels in normal melanocytes but was ectopically activated in approximately 10% of melanoma specimens.../profile/8773/arrayexpressuploader24cellmelanomaDec.12, 2014FalseE-GEOD-26603_A-AFFY-44ectopic-activation-of-arhgap8-in-melanoma-promoteshttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26603Ectopic activation of ARHGAP8 in melanoma promotes cell motilitySep.15, 2014ARHGAP8 was expressed at negligible levels in normal melanocytes but was ectopically activated in approximately 10% of melanoma specimens and melanoma cell lines. ARHGAP8 was ectopically expressed in ARHGAP8-negative M14 melanoma cells using the pIRES2 vector (Clontech), producing a bicistronic ARHGAP8-ires-eGFP mRNA. M14 cells stably transfected with vector control or N-terminally FLAG-tagged ARHGAP8 were cultured in 2D or 3D embedded growth conditions and then subjected to gene expression profiling using Affymetrix U133_Plus_2 gene chips. M14 cells stably transfected with pIRES2 alone or pIRES2 containing N-terminally FLAG-tagged ARHGAP8 were cultured in 2D or 3D conditions for 4 d as described. Total RNA was isolated using Trizol reagent (Invitrogen) and processed for application to Affymetrix gene chips.E-GEOD-26603http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26603/samples/