{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM654893": {"CULTURE CONDITION": "2D", "EXPRESSION PLASMID": "empty vector control (pIRES2)"}}, {"GSM654894": {"CULTURE CONDITION": "2D", "EXPRESSION PLASMID": "FLAG tagged ARHGAP8 in pIRES2"}}, {"GSM654895": {"CULTURE CONDITION": "3D", "EXPRESSION PLASMID": "empty vector control (pIRES2)"}}, {"GSM654896": {"CULTURE CONDITION": "3D", "EXPRESSION PLASMID": "FLAG tagged ARHGAP8 in pIRES2"}}], "id": 3923, "pop_total": 0, "platform": 4, "summary_wrapped": "ARHGAP8 was expressed at negligible levels in normal melanocytes but was ectopically activated in approximately 10% of melanoma specimens...", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 4, "tags": ["cell", "melanoma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-26603_A-AFFY-44", "slug": "ectopic-activation-of-arhgap8-in-melanoma-promotes", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26603", "geo_gds_id": "", "name": "Ectopic activation of ARHGAP8 in melanoma promotes cell motility", "created": "Sep.15, 2014", "summary": "ARHGAP8 was expressed at negligible levels in normal melanocytes but was ectopically activated in approximately 10% of melanoma specimens and melanoma cell lines. ARHGAP8 was ectopically expressed in ARHGAP8-negative M14 melanoma cells using the pIRES2 vector (Clontech), producing a bicistronic ARHGAP8-ires-eGFP mRNA. M14 cells stably transfected with vector control or N-terminally FLAG-tagged ARHGAP8 were cultured in 2D or 3D embedded growth conditions and then subjected to gene expression profiling using Affymetrix U133_Plus_2 gene chips. M14 cells stably transfected with pIRES2 alone or pIRES2 containing N-terminally FLAG-tagged ARHGAP8 were cultured in 2D or 3D conditions for 4 d as described. Total RNA was isolated using Trizol reagent (Invitrogen) and processed for application to Affymetrix gene chips.", "geo_gse_id": "E-GEOD-26603", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26603/samples/"}