{"platform": 6, "owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM653199 1": {}}, {"GSM653200 1": {}}, {"GSM653201 1": {}}, {"GSM653202 1": {}}, {"GSM653203 1": {}}, {"GSM653204 1": {}}], "id": 6325, "pop_total": 0, "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26568", "summary_wrapped": "On triggering of the T cell receptor CD8 T lymphocytes downregulate expression of the transcription factor KLF2.  KLF2 expression remains...", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 6, "tags": ["cell", "interleukin", "interleukin-2"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "impact-of-klf2-expression-on-t-cell-genetic-progra", "geo_id_plat": "E-GEOD-26568_A-AFFY-45", "name": "Impact of KLF2 expression on T cell genetic program", "created": "Nov.11, 2014", "summary": "On triggering of the T cell receptor CD8 T lymphocytes downregulate expression of the transcription factor KLF2.  KLF2 expression remains low as these cells differentiate to Cytotoxic T lymphocytes (CTL) but may be re-expressed depending on the local environmental signals. We used retroviral transduction to enforce KLF2 expression in CTL to determine the impact of it re-expression on the CTL genetic program. T lymphocytes with a transgenic T cell receptor (P14 LCMV) isolated from murine spleens were activated with gp33-41 peptide for 2 days and transduced with empty vector (evGFP) or GFP-KLF2.  After differentiation to CTL in culture with Interleukin-2 for 2 further days, cells positive for GFP were isolated by Fluorescence Activated Cell Sorting and the RNA extracted for microarray analysis.", "geo_gse_id": "E-GEOD-26568", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26568/samples/"}