{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3913, "factors": [{"GSM6514": {"phenotype": "naive"}}, {"GSM6514": {"phenotype": "naive"}}, {"GSM6514": {"phenotype": "naive"}}, {"GSM6514": {"phenotype": "naive"}}, {"GSM651416": {"phenotype": "high PD-1 expression"}}, {"GSM651416": {"phenotype": "high PD-1 expression"}}, {"GSM651416": {"phenotype": "high PD-1 expression"}}, {"GSM651416": {"phenotype": "high PD-1 expression"}}, {"GSM651416": {"phenotype": "high PD-1 expression"}}, {"GSM651416": {"phenotype": "high PD-1 expression"}}, {"GSM651423": {"phenotype": "low PD-1 expression"}}, {"GSM651423": {"phenotype": "low PD-1 expression"}}, {"GSM651423": {"phenotype": "low PD-1 expression"}}, {"GSM651423": {"phenotype": "low PD-1 expression"}}, {"GSM651423": {"phenotype": "low PD-1 expression"}}, {"GSM651423": {"phenotype": "low PD-1 expression"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "T cell dysfunction is an important feature of many chronic viral infections. In particular, it was shown that PD-1 regulates T cell...", "pubmed_id": 21383243, "geo_gse_id": "E-GEOD-26495", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 16, "tags": ["cell", "peripheral"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-human-cd8-posi", "geo_id_plat": "E-GEOD-26495_A-AFFY-44", "name": "Transcription profiling by array of human CD8-positive T cells with high or low programmed death-1 expression", "created": "Sep.15, 2014", "summary": "T cell dysfunction is an important feature of many chronic viral infections. In particular, it was shown that PD-1 regulates T cell dysfunction during chronic LCMV infection in mice and PD-1 high cells exhibit an intense exhausted gene signature. These findings were extended to human chronic infections such as HIV, HCV and HBV. However, it is not known if PD-1 high cells of healthy humans have the traits of exhausted cells. In this study, we provide a comprehensive description of phenotype, function and gene expression profiles of PD-1 high versus PD-1 low CD8 T cells in the peripheral blood of healthy human adults as following: 1) The percentage of naive and memory CD8 T cells varied widely in the peripheral blood cells of healthy humans and PD-1 was expressed by the memory CD8 T cells. 2) PD-1 high CD8 T cells in healthy humans did not significantly correlated with the PD-1 high exhausted gene signature of HIV specific human CD8 T cells or chronic LCMV specific CD8 T cells from mice. 3) PD-1 expression did not directly affect the ability of CD8 T cells to secrete cytokines in healthy adults. 4) PD-1 was expressed by the effector memory (TEM) compared to \u2018terminally differentiated effector\u2019 (TEMRA) CD8 T cells. 5) Finally, an interesting inverse relationship between CD45RA and PD-1 expression was observed. We used highly purified PD-1 high and PD-1 low from six healthy adult individuals and naive CD8 T cell populations from four of those individuals for gene expression studies", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26495", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26495/samples/"}