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Home › Dataset Library › Sirt1 targeting promotes Treg function

Dataset: Sirt1 targeting promotes Treg function

Sirtuin-1 (Sirt1), a class III histone/protein deacetylase is central to cellular metabolism, stress responses and aging, but its...

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Sirtuin-1 (Sirt1), a class III histone/protein deacetylase is central to cellular metabolism, stress responses and aging, but its contributions to various host immune functions have been little investigated. To study the role of Sirt1 in T-cell functions, we undertook targeted deletions by mating mice with a floxed Sirt1 gene to mice expressing CD4-cre or Foxp3-cre recombinase, respectively. We found that Sirt1 deletion left conventional T-effector cell activation, proliferation and cytokine production largely unaltered. However, Sirt1 targeting promoted the expression and acetylation of Foxp3, a key transcription factor in T-regulatory (Treg) cells, and increased Treg suppressive functions in vitro and in vivo. Consistent with these data, mice with targeted deletions of Sirt1 in either CD4+ T-cells or Foxp3+ Treg cells exhibited prolonged survival of MHC-mismatched cardiac allografts. Allografts in Sirt1 targeted recipients showed long-term preservation of myocardial histology and infiltration by Foxp3+ Treg cells. Comparable results were seen in wild-type allograft recipients treated with Sirt1 inhibitors, such as EX-527 and splitomicin. Hence, Sirt1 may inhibit Treg functions and its targeting may have therapeutic value in autoimmunity and transplantation. RNA from three independent samples from magnetically separated CD4+CD25+ Treg of Sirt1 knock out, compared to wild type (C57BL6) control

Species:
mouse

Samples:
9

Source:
E-GEOD-26425

Updated:
Dec.12, 2014

Registered:
Nov.24, 2014


Factors: (via ArrayExpress)
Sample GENOTYPE/VARIATION
GSM648480 Wild type
GSM648480 Wild type
GSM648480 Wild type
GSM648483 Conditional Sirt1 knockout (CD4 cre recombinase)
GSM648483 Conditional Sirt1 knockout (CD4 cre recombinase)
GSM648483 Conditional Sirt1 knockout (CD4 cre recombinase)
GSM648486 Conditional Sirt1 knockout (Foxp3 cre recombinase)
GSM648486 Conditional Sirt1 knockout (Foxp3 cre recombinase)
GSM648486 Conditional Sirt1 knockout (Foxp3 cre recombinase)

Tags

  • cell
  • central
  • class
  • cytokine
  • histone
  • left
  • protein

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